The Immune Response to Pneumococcal Polysaccharides 14 and 23F Among Elderly Individuals Consists Predominantly of Switched Memory B Cells
The Immune Response to Pneumococcal Polysaccharides 14 and 23F Among Elderly Individuals Consists Predominantly of Switched Memory B Cells
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DOI：
10.1093/infdis/jit139
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发表时间：
2013-07-01
影响因子：
6.4
通讯作者：
Westerink, M. A. Julie
中科院分区：
文献类型：
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作者：
Leggat, David J.;Thompson, Rebecca S.;Westerink, M. A. Julie
B细胞对纯化的肺炎球菌多糖（PPS）疫苗接种的反应表型一直是争论的话题。我们最近鉴定了来自健康年轻志愿者的细胞的表型为CD 27（+）IgM（+）B细胞。然而，PPS反应性B细胞群尚未在高风险人群（如老年人）中确定。以前的研究表明，老年人的免疫球蛋白M记忆B细胞的百分比低于健康的年轻人。在这项研究中，我们使用荧光标记的PPS 14和PPS 23 F直接表征了老年人接种PPS疫苗（PPV）前后PPS特异性B细胞的表型。与我们在健康年轻志愿者中的观察相反，PPS应答B细胞群主要由转换记忆（CD 27（+）IgM（-））B细胞组成。与这些结果一致，与年轻人相比，该人群接种后免疫球蛋白M浓度未显著增加，调理吞噬反应降低。这些发现确定了老年人中响应PPV的B细胞群体表型的显著变化。
The phenotype of B cells that respond to vaccination with the purified pneumococcal polysaccharide (PPS) has been a topic of debate. We have recently identified the phenotype of cells from healthy young volunteers as CD27(+)IgM(+) B cells. However, the PPS-responding B-cell population has not yet been identified in high-risk populations, such as elderly individuals. Previous studies have shown that elderly individuals have a lower percentage of immunoglobulin M memory B cells than healthy young adults. In this study, we directly characterized the phenotype of PPS-specific B cells before and after vaccination with PPS vaccine (PPV) in elderly adults, using fluorescently labeled PPS14 and PPS23F. In contrast to our observations in healthy young volunteers, the PPS-responding B-cell population consisted primarily of switched memory (CD27(+)IgM(-)) B cells. In concurrence with these findings, postvaccination immunoglobulin M concentrations were not significantly increased in this population, and the opsonophagocytic response was decreased, compared with that in young adults. These findings identify a significant shift in the phenotype of the B-cell population in response to PPV among elderly individuals.