2, 6-Dichlorobenzonitrile causes multiple effects on pollen tube growth beyond altering cellulose synthesis in Pinus bungeana Zucc.
2, 6-Dichlorobenzonitrile causes multiple effects on pollen tube growth beyond altering cellulose synthesis in Pinus bungeana Zucc.
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2, 6-二氯苯甲腈除了改变白皮松 Zucc 的纤维素合成之外，还对花粉管生长产生多重影响。
DOI：
10.1371/journal.pone.0076660
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Hao H;Chen T;Fan L;Li R;Wang X
纤维素是细胞壁的重要组成部分，但其在花粉管中的位置和功能仍是未知的。本文研究了纤维素合成在白皮松花粉管伸长过程中的作用。用特异性抑制剂2，6-二氯苯腈（DCB）进行抑制。在DCB存在下，花粉管的生长速度和形态发生了明显的变化。细胞骨架和囊泡运输的组织也受到干扰。DCB处理后的花粉管超微结构表现为管壁疏松、细胞器受损。DCB处理引起管壁成分的明显变化。荧光标记结果表明，胼胝质和酸性果胶积累在尖端区域，而有较少的纤维素处理DCB。这些结果得到了FTIR显微光谱分析的证实。总之，我们的研究结果表明，DCB抑制纤维素合成影响细胞骨架的组织和小泡运输在花粉管中，并诱导管壁化学组成的变化，在剂量依赖性的方式。这些结果证实了纤维素参与了花粉管生长方向的确立，并在花粉管发育过程中对细胞壁的构建起着重要作用，尽管纤维素含量较低。
Cellulose is an important component of cell wall, yet its location and function in pollen tubes remain speculative. In this paper, we studied the role of cellulose synthesis in pollen tube elongation in Pinus bungeana Zucc. by using the specific inhibitor, 2, 6-dichlorobenzonitrile (DCB). In the presence of DCB, the growth rate and morphology of pollen tubes were distinctly changed. The organization of cytoskeleton and vesicle trafficking were also disturbed. Ultrastructure of pollen tubes treated with DCB was characterized by the loose tube wall and damaged organelles. DCB treatment induced distinct changes in tube wall components. Fluorescence labeling results showed that callose, and acidic pectin accumulated in the tip regions, whereas there was less cellulose when treated with DCB. These results were confirmed by FTIR microspectroscopic analysis. In summary, our findings showed that inhibition of cellulose synthesis by DCB affected the organization of cytoskeleton and vesicle trafficking in pollen tubes, and induced changes in the tube wall chemical composition in a dose-dependent manner. These results confirm that cellulose is involved in the establishment of growth direction of pollen tubes, and plays important role in the cell wall construction during pollen tube development despite its lower quantity.