A rapid method for isolation of low-molecular-weight RNA from Arabidopsis using low salt concentration buffer.
A rapid method for isolation of low-molecular-weight RNA from Arabidopsis using low salt concentration buffer.
复制标题
一种使用低盐浓度缓冲液从拟南芥中快速分离低分子量RNA的方法
DOI：
10.4081/pb.2010.e14
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发表时间：
2010-01-01
影响因子：
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通讯作者：
Huang, H. S.
中科院分区：
文献类型：
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作者：
Cheng Han, Cheng Han;Gao Jing, Gao Jing;Huang, H. S.
正常0 7.8 pt 0 2假假假EN-US ZH-CN X-NONE MicrosoftInternetExplorer 4我们建立了一种利用低盐缓冲液快速提取拟南芥组织中低分子量RNA的方法。该方法快速、高效，小规模提取过程不超过1小时，而RNA产量和质量与以前报道的相当。该方法提取的低分子量RNA质量高，小分子量RNA含量丰富，不含高分子量RNA。该方法可用于小分子RNA的克隆、检测和文库构建。
Normal 0 7.8 pt 0 2 false false false EN-US ZH-CN X-NONE MicrosoftInternetExplorer4 We have developed a rapid extraction method using low salt concentration buffer for the isolation of low-molecular-weight RNA from Arabidopsis tissues. The method was quick and efficient, and the small scale extraction process took no more than 1 hour, while yield and RNA quality were comparable with those of previously reported. The LMW RNA isolated using this method was high quality, abundant in small RNA and free of high molecular weight RNA. This method can be used to extract low-molecular-weight RNA for the purpose of small RNA cloning and detection, and library construction.