Whole Genome Sequencing Allows Better Understanding of the Evolutionary History of Leptospira interrogans Serovar Hardjo.
Whole Genome Sequencing Allows Better Understanding of the Evolutionary History of Leptospira interrogans Serovar Hardjo.
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DOI：
10.1371/journal.pone.0159387
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Rajeev S
中科院分区：
文献类型：
--
作者：
Llanes A;Restrepo CM;Rajeev S
对问号钩端螺旋体Hardjo血清型实验室适应株的基因组进行了测序和分析。与最近公布的相同血清型的田间分离株的测序基因组的比较显示在核苷酸水平上相对较高的序列保守性，尽管两种样品的生物学背景不同。相反，两个血清型Hardjo基因组与L。Borgpetersenii血清型Hardjo在相应的染色体之间显示出广泛的差异，除了它们的rfb基因座所占据的区域。此外，还比较了Hardjo血清型与不同L.问号线虫血清型使我们能够检测到几个基因组特征，这些特征可能赋予L.问号血清型Hardjo，包括可能的整合质粒和编码已知参与耐药性的膜转运系统的簇的额外拷贝。为了更好地了解Hardjo血清型在L.问号血清型和其他钩端螺旋体属物种。提出的同源性支持这一假设，即在两个不同的物种中存在相似的rfb基因座可能是横向基因转移事件的结果。
The genome of a laboratory-adapted strain of Leptospira interrogans serovar Hardjo was sequenced and analyzed. Comparison of the sequenced genome with that recently published for a field isolate of the same serovar revealed relatively high sequence conservation at the nucleotide level, despite the different biological background of both samples. Conversely, comparison of both serovar Hardjo genomes with those of L. borgpetersenii serovar Hardjo showed extensive differences between the corresponding chromosomes, except for the region occupied by their rfb loci. Additionally, comparison of the serovar Hardjo genomes with those of different L. interrogans serovars allowed us to detect several genomic features that may confer an adaptive advantage to L. interrogans serovar Hardjo, including a possible integrated plasmid and an additional copy of a cluster encoding a membrane transport system known to be involved in drug resistance. A phylogenomic strategy was used to better understand the evolutionary position of the Hardjo serovar among L. interrogans serovars and other Leptospira species. The proposed phylogeny supports the hypothesis that the presence of similar rfb loci in two different species may be the result of a lateral gene transfer event.