Formation of cubic phases from large unilamellar vesicles of dioleoylphosphatidylglycerol/monoolein membranes induced by low concentrations of Ca2+
Formation of cubic phases from large unilamellar vesicles of dioleoylphosphatidylglycerol/monoolein membranes induced by low concentrations of Ca2+
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DOI：
10.1021/la051782i
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发表时间：
2005-12-06
期刊：
影响因子：
3.9
通讯作者：
Yamazaki, M
中科院分区：
文献类型：
--
作者：
Awad, TS;Okamoto, Y;Yamazaki, M
我们开发了一种将大单层囊泡（LUVs）转化为立方相的新方法。我们发现，在单油（MO）和二油基磷脂酰甘油（DOPG）混合物（DOPG /MO）膜的多层囊泡（MLVs）悬浮液中加入低浓度的Ca2+使其l相变为立方相。例如，添加15-25毫米的Ca2+到。30%-DOPG/ 70%-MO-MLVs诱导Q（229）相，而添加>= 28 mM Ca2+诱导Q（224）相。含>= 25 mol% DOPG的DOPG/MO膜易于制备。低浓度的Ca2+将过量缓冲液中的luv转化为Q（224）或Q（229）相，具体取决于Ca2+浓度。例如，在25℃下，15和50 mM Ca2+分别诱导了30%-DOPG/ 70%- mo - luv的Q（224）和Q（229）相。这一发现是首次证明脂质膜的LLTVs在过量水条件下转化为立方相。
We developed a new method for the transformation of large unilamellar vesicles (LUVs) into the cubic phase. We found that the addition of low concentrations of Ca2+ to suspensions of multilamellar vesicles (MLVs) of membranes of monoolein (MO) and dioleoylphosphatidylglycerol (DOPG) mixtures I DOPG/MO) changed their L. phase to the cubic phases. For instance, the addition of 15-25 mM Ca2+ to.30%-DOPG/ 70%-MO-MLVs induced the Q(229) phase, whereas the addition of >= 28 mM Ca2+ induced the Q(224) phase. LUVs of DOPG/MO membranes containing >= 25 mol% DOPG were prepared easily. Low concentrations of Ca2+ transformed these LUVs in excess buffer into the Q(224) or the Q(229) phase, depending on the Ca2+ concentration. For example, 15 and 50 mM Ca2+ induced the Q(224) and Q(229) phase in the 30%-DOPG/ 70%-MO-LUVs at 25 degrees C, respectively. This finding is the first demonstration or transformation of LLTVs of lipid membranes into the cubic phase under excess water condition.