Reduced Expression of PER3 Is Associated with Incidence and Development of Colon Cancer
Reduced Expression of PER3 Is Associated with Incidence and Development of Colon Cancer
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PER3 表达降低与结肠癌的发病和发展相关
DOI：
10.1245/s10434-012-2279-5
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发表时间：
2012-09-01
影响因子：
3.7
通讯作者：
Peng, Zhihai
中科院分区：
文献类型：
--
作者：
Wang, Xiaoliang;Yan, Dongwang;Peng, Zhihai
Period 3(Period 3，PER3)是一种昼夜节律调节蛋白，影响细胞周期、生长和分化。方法通过建立基因表达长序列分析(SAGE)数据库，采用实时定量聚合酶链式反应(RT-PCR)和免疫组织化学方法，分析PER3在结肠癌患者正常组织和癌组织中的表达。结果与正常组织相比，结肠癌组织中PER3的表达水平降低了2.8倍。实时荧光定量聚合酶链式反应分析显示，结肠癌和直肠肿瘤患者肿瘤组织中PER3mRNA水平均低于正常组织(P<；P<0.001)。此外，PER3的表达与多种临床病理因素有关，包括肿瘤的部位、分化程度和分期。此外，PER3阴性组的死亡率较高(P=0.025)，PER3阳性组和PER3阴性组总生存期分别为71.5±22.2月和58.6%±15.0月(P=0.020)。结论PER3可能在结肠癌的进展中起作用。
BackgroundPeriod 3 (PER3), a circadian regulation protein, influences cell cycle, growth, and differentiation. The aim of the present study was to determine whether PER3 expression is associated with colon cancer incidence and progression.MethodsPER3 expression was analyzed in the normal and cancerous tissues from patients with colon cancer by establishing a long serial analysis of gene expression (SAGE) database as well as by real-time PCR and immunohistochemistry.ResultsAs compared with normal tissue, a 2.8-fold decrease in PER3 mRNA levels in colon cancerous tissue was observed. Real-time PCR analysis revealed that PER3 mRNA levels in tumor tissues were lower than in normal tissues (P< 0.001) in both patients with colon tumor and those with rectal tumor. In addition, PER3 expression was related to multiple clinicopathologic factors, including tumor location, differentiation, and stage. Furthermore, the incidence of death was higher in subjects with PER3-negative tumors (P= 0.025); the estimated overall survival time was 71.5 ± 2.2 months and 58.6 ± 5.0 months in subjects with PER3-positive and PER3-negative tumors, respectively (P= 0.020).ConclusionsPER3 may play a role in colon cancer progression.