Distinct patterns of cell motion inside a micro-channel under different osmotic conditions
Distinct patterns of cell motion inside a micro-channel under different osmotic conditions
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不同渗透条件下微通道内细胞运动的不同模式
DOI：
10.1109/embc.2013.6610801
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
F. Arai
中科院分区：
文献类型：
--
作者：
C. Tsai;M. Kaneko;S. Sakuma;F. Arai
本文首次研究了微通道内渗透条件对活细胞的影响。利用高速摄像机，我们观察到不同渗透条件下细胞运动的不同模式，这些模式是由不同浓度的氯化钠（NaCl）盐水建立的。细胞运动由计算机跟踪，并以位置和时间坐标（x-t图）表示。低渗条件下（NaCl% <; 0.9%）细胞的运动曲线为凸曲线，等渗和高渗条件下（NaCl%≥0.9%）细胞的运动曲线为凹曲线。由于生理盐水在医学实践和细胞相关研究中的广泛应用，我们的研究结果指出了两个重要事实：1)细胞对NaCl的百分比敏感。1%的总浓度差异会使细胞特性发生巨大变化，例如细胞硬度。2)微通道法可以根据细胞的运动规律清晰地分辨出低渗、等渗和高渗状态的差异。本文还从不同的角度对这一现象进行了解释。
The effect of osmotic condition on a living cell inside a micro-channel is firstly studied in this work. By utilizing a high-speed camera, we observed distinct patterns of cell motion under different osmotic conditions, which are established by saline with different concentrations of sodium chloride (NaCl). The cell motions are tracked by a computer, and are presented by the coordinates of location and time (x-t chart). The motions of cells under hypotonic condition (NaCl% <; 0.9%) are convex curves on the chart while the ones under isotonic and hypertonic conditions (NaCl% ≥ 0.9%) are concave curves. Since saline is widely used in both medical practices and cell-related researches, our results point out two important facts: 1) Cells are sensitive to the percentage of NaCl. One percent difference in overall concentration makes dramatic changes in cell characteristics, such as cell stiffness. 2) The micro-channel method can clearly tell the difference between hypotonic, isotonic and hypertonic conditions according to the pattern of cell motion. Interpretations of the phenomena from different perspectives are also discussed in this paper.