AgarTrap Protocols on your Benchtop: Simple Methods for Agrobacterium-mediated Genetic Transformation of the Liverwort Marchantia polymorpha.
AgarTrap Protocols on your Benchtop: Simple Methods for Agrobacterium-mediated Genetic Transformation of the Liverwort Marchantia polymorpha.
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台式 AgarTrap 实验方案：农杆菌介导的地钱遗传转化的简单方法。
作者：
Shoko Tsuboyama;Y. Kodama
农杆菌介导的遗传转化是植物生物学研究中的一项重要技术。我们最近开发了一种简化的农杆菌介导的遗传转化方法地钱多形物，命名为AgarTrap（琼脂利用转化倾倒解决方案）。AgarTrap很容易执行;所有的程序都可以在一周内完成，使用一个固体培养基的平板，并且基本操作包括简单地将适当的溶液倾倒到固体培养基上。到目前为止，我们已经开发了三种类型的AgarTrap方法（S-AgarTrap，G-AgarTrap和T-AgarTrap），使用三种不同的M。polymorpha组织：孢子、完整的萌发和成熟的叶状体碎片。每种AgarTrap方法都可以用于高效转化组织，从而产生足够数量的转化体用于研究。这些AgarTrap方法的简便性和有效性将可能促进M.多形。在这篇综述中，我们描述了三种AgarTrap方法的基本特征，并介绍了我们实验室使用的详细方案。
Agrobacterium-mediated genetic transformation is a powerful technique in plant biology. We recently developed a simplified Agrobacterium-mediated genetic transformation method for the liverwort Marchantia polymorpha, named AgarTrap (agar-utilized transformation with pouring solutions). AgarTrap is easy to perform; all procedures can be completed within a week using a single plate of solid medium, and basic operations involve simply pouring the appropriate solutions onto the solid medium. Thus far, we have developed three types of AgarTrap methods (S-AgarTrap, G-AgarTrap, and T-AgarTrap) using three different M. polymorpha tissues: sporelings, intact gemmalings, and mature thallus pieces, respectively. Each AgarTrap method can be used to transform tissues at high efficiency, thereby producing sufficient numbers of transformants for study. The ease and efficiency of these AgarTrap methods will likely prompt widespread molecular biological analyses of M. polymorpha. In this review, we describe the basic characteristics of the three AgarTrap methods and present the detailed protocols used in our laboratory.
影响因子：
64.5
作者：
Bowman, John L.;Kohchi, Takayuki;Schmutz, Jeremy
通讯作者：
Schmutz, Jeremy