Histopathological features of condylar hyperplasia and condylar Osteochondroma: a comparison study
Histopathological features of condylar hyperplasia and condylar Osteochondroma: a comparison study
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髁突增生与髁突骨软骨瘤组织病理学特征的比较研究
DOI：
10.1186/s13023-019-1272-5
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发表时间：
2019-12-16
影响因子：
3.7
通讯作者：
Wang, Xudong
中科院分区：
文献类型：
--
作者：
Yu, Jingshuang;Yang, Tong;Wang, Xudong
研究背景虽然下颌骨的生物学行为不同，但两者均可导致颌面部骨骼不对称和错(牙合)。本研究试图用苏木精-伊红(H&E)染色和免疫组织化学染色结合定量分析的方法，比较下颌骨髁状突增生症和髁状突骨软骨瘤的组织学特征。髁状突骨软骨瘤的软骨帽厚度明显高于单纯增生(P= 0.018)。髁状突骨软骨瘤的成骨百分率高于单纯增生(P= 0.0 4)。免疫组织化学染色显示，增殖细胞核抗原主要分布在未分化间充质层和肥大的软骨层，其中在骨软骨瘤中增殖细胞核抗原阳性细胞较多(P= 0.007)。Ext1主要表达于软骨层，在骨软骨瘤中也有较高的阳性表达(P= 0.0366)。软骨帽越厚，骨形成率越高，增殖细胞核抗原阳性率越高，说明骨软骨瘤的增殖率越高。结论EXT1在骨软骨瘤和增生性骨软骨瘤中的表达差异有统计学意义，其阳性率明显高于增生性骨软骨瘤。
BackgroundBoth mandibular condylar hyperplasia and condylar osteochondroma can lead to maxillofacial skeletal asymmetry and malocclusion, although they exhibit different biological behavior. This study attempted to compare the histological features of mandibular condylar hyperplasia and condylar osteochondroma using hematoxylin-and-eosin (H&E) staining, and immunohistochemistry staining of PCNA and EXT1 with quantitative analysis method.ResultsThe H&E staining showed that condylar hyperplasia and condylar osteochondroma could be divided into four histological types and exhibited features of different endochondral ossification stages. There was evidence of a thicker cartilage cap in condylar osteochondroma as compared condylar hyperplasia (P= 0.018). The percentage of bone formation in condylar osteochondroma was larger than was found in condylar hyperplasia (P= 0.04). Immunohistochemical staining showed that PCNA was mainly located in the undifferentiated mesenchymal layer and the hypertrophic cartilage layer, and there were more PCNA positive cells in the condylar osteochondroma (P= 0.007). EXT1 was mainly expressed in the cartilage layer, and there was also a higher positive rate of EXT1 in condylar osteochondroma (P= 0.0366). The thicker cartilage cap, higher bone formation rate and higher PCNA positive rate indicated a higher rate of proliferative activity in condylar osteochondroma. The more significant positive rate of EXT1 in condylar osteochondroma implied differential biological characteristic as compared to condylar hyperplasia.ConclusionsThese features might be useful in histopathologically distinguishing condylar hyperplasia and osteochondroma.