Frog virus 3 prevalence in tadpole populations inhabiting cattle-access and non-access wetlands in Tennessee, USA
Frog virus 3 prevalence in tadpole populations inhabiting cattle-access and non-access wetlands in Tennessee, USA
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DOI：
10.3354/dao01837
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发表时间：
2007-09-14
影响因子：
1.4
通讯作者：
Baldwin, Charles A.
中科院分区：
文献类型：
--
作者：
Gray, Matthew J.;Miller, Debra L.;Baldwin, Charles A.
在美国，蛙病毒与大多数报道的无尾两栖类幼虫死亡有关。据推测，病原体引起的压力可能会增加两栖动物种群的病原体患病率，损害免疫力。牛对湿地的使用可能会通过降低水质来给蝌蚪种群带来压力。从绿色蛙（Rana clamitans）和美国牛蛙（R.在美国田纳西州坎伯兰高原的5个牛可进入湿地和3个非可进入湿地采集的catesbeiana（n = 104）蝌蚪。测序证实青蛙病毒3（FV3），因此，我们比较了其流行率之间的蝌蚪种群栖息牛访问和非访问湿地，并在3个季节（冬季，夏季和秋季）在2005年。我们发现FV3的蝌蚪物种和牛的土地利用类型，然而，患病率FV3是更大的绿色青蛙蝌蚪居住在牛访问湿地相比，在非访问湿地。在美国牛蛙蝌蚪的牛用地之间检测到FV3的患病率没有差异。FV3流行率也存在季节性趋势，两个物种秋季和冬季的流行率高于夏季。此外，我们发现在美国牛蛙蝌蚪中，随着Gosner分期的增加，FV 3的患病率显著降低。绿色蛙蝌蚪的FV3感染率与其发育阶段之间没有明显的相关性。我们的研究结果表明，牛使用湿地可能会增加患病率的FV3蛙蝌蚪，虽然这种影响可能取决于物种，季节和蝌蚪的发育阶段。
Ranaviruses have been associated with most of the reported larval anuran die-offs in the United States. It is hypothesized that anthropogenically induced stress may increase pathogen prevalence in amphibian populations by compromising immunity. Cattle use of wetlands may stress resident tadpole populations by reducing water quality. We isolated a Ranavirus from green frog Rana clamitans (n = 80) and American bullfrog R. catesbeiana (n = 104) tadpoles collected at 5 cattle-access and 3 non-access wetlands on the Cumberland Plateau, Tennessee, USA. Sequencing confirmed Frog virus 3 (FV3); therefore, we compared its prevalence between tadpole populations inhabiting cattle-access and non-access wetlands, and among 3 seasons (winter, summer, and autumn) in 2005. We found FV3 in both tadpole species and cattle land-use types; however, prevalence of FV3 was greater in green frog tadpoles residing in cattle-access wetlands compared to those in non-access wetlands. No difference in FV3 prevalence was detected between cattle land uses for American bullfrog tadpoles. A seasonal trend in FV3 prevalence also existed, with prevalence greater in autumn and winter than in summer for both species. In addition, we found that FV3 prevalence decreased significantly as Gosner stage increased in American -bullfrog tadpoles. No trend was detected between FV3 prevalence and developmental stage for green frog tadpoles. Our results suggest that cattle use of wetlands may increase prevalence of FV3 in Rana tadpoles, although this effect may depend on species, season, and tadpole developmental stage.