Rhinovirus increases human β-defensin-2 and -3 mRNA expression in cultured bronchial epithelial cells
Rhinovirus increases human β-defensin-2 and -3 mRNA expression in cultured bronchial epithelial cells
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DOI：
10.1016/s0928-8244(03)00106-8
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发表时间：
2003-08-18
影响因子：
--
通讯作者：
Hiemstra, PS
中科院分区：
文献类型：
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作者：
Duits, LA;Nibbering, PH;Hiemstra, PS
人β -防御素（hBDs）是一种抗菌肽，在宿主防御感染、炎症和免疫中起重要作用。先前的研究表明，微生物和促炎介质调节这些肽在气道上皮细胞中的表达。本研究的目的是研究鼻病毒-16 （RV16）对培养的原代支气管上皮细胞（PBEC）中hBDs表达的调节，鼻病毒-16是一种引起普通感冒并与哮喘加重相关的呼吸道病毒。RV16可诱导PBEC中hBD-2和-3 mRNA的表达，但不影响hBD-1 mRNA的表达。病毒复制似乎是鼻病毒诱导的β -防御素mRNA表达的必要条件，因为紫外线灭活的鼻病毒不会增加hBD-2和hBD-3 mRNA的表达。暴露于合成双链RNA （dsRNA）分子多肌苷：多胞酸对PBEC中这些肽的mRNA表达的影响与RV16相似。与此相一致，PBEC被发现表达TLR3，一种参与识别dsRNA的toll样受体。本研究表明，鼻病毒感染PBEC导致hBD-2和hBD-3 mRNA表达增加，这可能在无并发症的普通感冒和病毒相关的哮喘加重中发挥作用。(C) 2003欧洲微生物学会联合会。Elsevier Science B.V.版权所有。
Human beta-defensins (hBDs) are antimicrobial peptides that play important roles in host defense against infection, inflammation and immunity. Previous studies showed that micro-organisms and proinflammatory mediators regulate the expression of these peptides in airway epithelial cells. The aim of the present study was to investigate the modulation of expression of hBDs in cultured primary bronchial epithelial cells (PBEC) by rhinovirus-16 (RV16), a respiratory virus responsible for the common cold and associated with asthma exacerbations. RV16 was found to induce expression of hBD-2 and -3 mRNA in PBEC, but did not affect hBD-1 mRNA. Viral replication appeared essential for rhinovirus-induced beta-defensin mRNA expression, since UV-inactivated rhinovirus did not increase expression of hBD-2 and hBD-3 mRNA. Exposure to synthetic double-stranded RNA (dsRNA) molecule polyinosinic:polycytidylic acid had a similar effect as RV16 on mRNA expression of these peptides in PBEC. In line with this, PBEC were found to express TLR3, a Toll-like receptor involved in recognition of dsRNA. This study shows that rhinovirus infection of PBEC leads to increased hBD-2 and hBD-3 mRNA expression, which may play a role in both the uncomplicated common cold and in virus-associated exacerbations of asthma. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.