The Anticancer Role of Capsaicin in Experimentallyinduced Lung Carcinogenesis.
The Anticancer Role of Capsaicin in Experimentallyinduced Lung Carcinogenesis.
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辣椒素在实验诱导的肺癌发生中的抗癌作用。
DOI：
10.3831/kpi.2015.18.011
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发表时间：
2015-06
影响因子：
1.4
通讯作者：
Devaki T
中科院分区：
文献类型：
--
作者：
Anandakumar P;Kamaraj S;Jagan S;Ramakrishnan G;Asokkumar S;Naveenkumar C;Raghunandhakumar S;Vanitha MK;Devaki T
辣椒素(CAP)是辣椒和辣椒中的主要辛辣成分，长期以来，辣椒一直被用作香料、食品添加剂和药物。本研究通过在小鼠肺癌发生过程中修饰细胞外基质成分和蛋白水解酶的能力来研究CAP的抗癌潜力。给瑞士白化小鼠灌胃苯并(A)芘(50 mg/kg体重，溶于橄榄油)，每周2次，连续4周，于第14周末诱发肺癌。卡铂(10 mg/kg体重，溶于橄榄油)腹腔注射。测定肺组织细胞外基质成分；肺组织进行Masson‘s毛染色。同时对基质金属蛋白酶2和9进行了蛋白质印迹分析。与对照动物相比，苯并(A)芘诱发肺癌的动物细胞外基质成分如胶原(羟脯氨酸)、弹性蛋白、糖醛酸和氨基己糖显著增加，糖胺聚糖如透明质酸、硫酸软骨素、硫酸角蛋白和硫酸皮肤素显著增加。与单独添加苯并(A)芘的动物相比，添加CAP的苯并(A)芘可有效地抵消上述细胞外基质成分的改变。马森的胶原毛染色和基质金属蛋白酶2和9的免疫印迹分析的结果进一步支持了生化结果。CAP在调节细胞外基质成分和蛋白酶方面的明显潜力表明，CAP具有化学调节和抗癌作用，可对抗实验诱导的肺癌发生。
Capsaicin (CAP) is the chief pungent principle found in the hot red peppers and the chili peppers that have long been used as spices, food additives and drugs. This study investigated the anticancer potential of CAP through its ability to modify extracellular matrix components and proteases during mice lung carcinogenesis. Swiss albino mice were treated with benzo(a) pyrene (50 mg/kg body weight dissolved in olive oil) orally twice a week for four successive weeks to induce lung cancer at the end of 14th week. CAP was administrated (10 mg/kg body weight dissolved in olive oil) intraperitoneally. Extracellular matrix components were assayed; Masson’s trichome staining of lung tissues was performed. Western blot analyses of matrix metalloproteases 2 and 9 were also carried out. In comparison with the control animals, animals in which benzo(a)pyrene had induced lung cancer showed significant increases in extracellular matrix components such as collagen (hydroxy proline), elastin, uronic acid and hexosamine and in glycosaminoglycans such as hyaluronate, chondroitin sulfate, keratan sulfate and dermatan sulfate. The above alterations in extracellular matrix components were effectively counteracted in benzo(a)pyrene along with CAP supplemented animals when compared to benzo(a) pyrene alone supplemented animals. The results of Masson’s trichome staining for collagen and of, immunoblotting analyses of matrix metalloproteases 2 and 9 further supported the biochemical findings. The apparent potential of CAP in modulating extracellular matrix components and proteases suggests that CAP plays a chemomodulatory and anti- cancer role working against experimentally induced lung carcinogenesis.