An efficient and non-enzymatic method for isolation and culture of endothelial cells from the nidus of human cerebral arteriovenous malformations
An efficient and non-enzymatic method for isolation and culture of endothelial cells from the nidus of human cerebral arteriovenous malformations
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DOI：
10.1016/j.neulet.2013.05.050
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发表时间：
2013-08-26
影响因子：
2.5
通讯作者：
Xu, Zai-Hua
中科院分区：
文献类型：
--
作者：
Zhang, Hai-Feng;Liang, Guo-Biao;Xu, Zai-Hua
在本报告中，我们描述了一种有效的非酶方法，用于从手术切除的动静脉畸形（AVM）标本的病灶中分离和培养内皮细胞（EC）。这些培养的细胞具有典型的表型标记（即血管性血友病因子和 CD34），以及 EC 的形态和超微结构特征。然而，他们激活了 Notch-1 信号传导，该信号在 AVM 的发展中发挥着关键作用。本研究表明，来自人脑动静脉畸形（CAVMEC）病灶的缺氧内皮细胞具有血管生成潜力，因为我们的数据显示，随着长时间缺氧，VEGF 基因表达和细胞增殖更加明显。在我们的研究中，我们成功地采用血管组织外植体贴壁法分离培养高纯度的CAVMEC。这可能被证明是研究介导 AVM 异常血管发育和维持的分子机制的有用工具。 (C) 2013 Elsevier Ireland Ltd. 保留所有权利。
In this report, we describe an efficient and non-enzymatic method for isolating and culturing endothelial cells (ECs) from the nidus of surgically resected arteriovenous malformation (AVM) specimens. These cultured cells possessed typical phenotypic markers (i.e. von Willebrand factor and CD34), as well as morphological and ultrastructural characteristics of ECs. However, they had activated Notch-1 signaling, which plays a critical role in the development of AVM. The present study suggests that hypoxic endothelial cells from the nidus of human cerebral arteriovenous malformation (CAVMECs) have angiogenic potentials, as our data showed that VEGF gene expression and cell proliferation were more evident with prolonged hypoxia. In our study, we successfully used the vascular tissue explants adherent method to isolate and culture CAVMECs with high purity. This may prove to be a useful tool for studying the molecular mechanisms that mediate abnormal vessel development and maintenance in AVM. (C) 2013 Elsevier Ireland Ltd. All rights reserved.