SynCAM, a synaptic adhesion molecule that drives synapse assembly
SynCAM, a synaptic adhesion molecule that drives synapse assembly
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DOI：
10.1126/science.1072356
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发表时间：
2002-08-30
期刊：
影响因子：
56.9
通讯作者：
Südhof, TC
中科院分区：
文献类型：
--
作者：
Biederer, T;Sara, Y;Südhof, TC
突触是神经细胞之间的连接，它们通过突触前和突触后的特化进行协调组装和紧密连接而形成。我们现在发现SynCAM是一种脑特异的含有免疫球蛋白结构域的蛋白，它与细胞内的PDZ结构域蛋白结合，在突触处起到亲水性细胞黏附分子的作用。分离的SynCAM胞浆尾巴在神经元中的表达抑制了突触组装。相反，在非神经元细胞中表达全长SynCAM可以诱导具有正常释放特性的海马神经元共同培养的突触形成。通过与SynCAM共表达谷氨酸受体，在这些非神经元细胞中重建了谷氨酸能突触传递，这表明单一类型的黏附分子和谷氨酸受体足以实现功能性的突触后反应。
Synapses, the junctions between nerve cells through which they communicate, are formed by the coordinated assembly and tight attachment of pre- and postsynaptic specializations. We now show that SynCAM is a brain-specific, immunoglobulin domain containing protein that binds to intracellular PDZ-domain proteins and functions as a homophilic cell adhesion molecule at the synapse. Expression of the isolated cytoplasmic tail of SynCAM in neurons inhibited synapse assembly. Conversely, expression of full-length SynCAM in nonneuronal cells induced synapse formation by cocultured hippocampal neurons with normal release properties. Glutamatergic synaptic transmission was reconstituted in these nonneuronal cells by coexpressing glutamate receptors with SynCAM, which suggests that a single type of adhesion molecule and glutamate receptor are sufficient for a functional postsynaptic response.