Peak frequencies of circulating human influenza-specific antibody secreting cells correlate with serum antibody response after immunization
Peak frequencies of circulating human influenza-specific antibody secreting cells correlate with serum antibody response after immunization
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DOI：
10.1016/j.vaccine.2010.02.088
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发表时间：
2010-04-30
期刊：
影响因子：
5.5
通讯作者：
Lee, F. Eun-Hyung
中科院分区：
文献类型：
--
作者：
Halliley, Jessica L.;Kyu, Shuya;Lee, F. Eun-Hyung
接种后，B细胞分化为抗体分泌细胞（ASC），其通过循环迁移到组织。这种反应的动力学和循环ASCs与保护性抗体滴度的关系尚未完全探索。方法：在三价流感疫苗接种（TIV）后，通过Elispot和流式细胞术每天对6名健康成人血液中的流感特异性和总IgG ASCs进行计数。峰值H1特异性IgG ASC频率在第5天至第8天出现，并与血凝抑制（HAI滴度）的倍数上升相关; r=0.91，p=0.006。H3特异性IgG ASC频率相关性较差，可能是由于疫苗中的H3蛋白与Elispot测定中使用的H3蛋白不匹配。疫苗特异性和总IgG ASC的峰值频率分别为外周血单核细胞（PBMC）的0.3%和0.8%。峰值TIV-、H1-、H3-和总IgG ASC频率分别为1736 +/- 1133、626 +/- 520、592 +/- 463和4091 +/- 2019个斑点/10（6）PBMC。TIV-、H1-和H3-特异性IgG ASC占总IgG ASC频率的峰值分别为63% +/- 21、26% +/- 10、22% +/-17.Conclusion：用灭活流感疫苗免疫后，流感特异性ASC频率的峰值是可变的，但与保护性HAI应答的幅度相关。(C)2010爱思唯尔有限公司保留所有权利。
Upon vaccination, B cells differentiate into antibody secreting cells (ASCs) that migrate via the circulation to tissues. The kinetics of this response and the relationship of circulating ASCs to protective antibody titers have not been completely explored.Methods: Influenza-specific and total-IgG ASCs were enumerated by Elispot and flow cytometry daily in the blood in 6 healthy adults after trivalent influenza vaccination (TIV).Results: Peak H1-specific IgG ASC frequencies occurred variably from day 5 to 8 and correlated with the fold-rise rise in hemagglutination inhibition (HAI titers); r=0.91, p=0.006. H3-specific IgG ASC frequencies correlated less well, perhaps due to a mismatch of the H3 protein in the vaccine and that used in the Elispot assay. Peak frequencies of vaccine-specific and total-IgG ASCs were 0.3% and 0.8%, respectively, of peripheral blood mononuclear cells (PBMC). Peak TIV-, H1-, H3-, and total-IgG ASC frequencies were 1736 +/- 1133, 626 +/- 520, 592 +/- 463, and 4091 +/- 2019 spots/10(6) PBMC, respectively. Peak TIV-, H1-, and H3-specific IgG ASC of total-IgG ASC frequencies constituted 63% +/- 21, 26% +/- 10, 22% +/- 17, respectively.Conclusion: After immunization with inactivated influenza vaccine the peak in influenza-specific ASC frequencies is variable but correlates well with the magnitude of protective HAI responses. (C) 2010 Elsevier Ltd. All rights reserved.