A mutation in protein phosphatase 2A regulatory subunit A affects auxin transport in Arabidopsis
A mutation in protein phosphatase 2A regulatory subunit A affects auxin transport in Arabidopsis
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DOI：
10.1002/j.1460-2075.1996.tb00565.x
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发表时间：
1996-05-01
期刊：
影响因子：
11.4
通讯作者：
Soll, D
中科院分区：
文献类型：
--
作者：
Garbers, C;DeLong, A;Soll, D
植物激素生长素控制细胞伸长、根毛发育和根分枝等过程，趋向性、重力、光和触觉触发的生长曲率也是生长素介导的反应，生长素在茎尖合成并通过茎转运，但生长素转运的分子机制尚不清楚，萘基邻苯二甲酸（NPA）和其他生长素转运抑制剂阻断趋向性曲率反应并抑制根和芽伸长，我们分离出一种新的拟南芥突变体，命名为 NPA 中的根卷曲 (rcn1)，突变体幼苗在根卷曲和下胚轴伸长方面表现出对 NPA 的反应改变，突变体幼苗中的生长素外流表现出对 NPA 的敏感性增加。 rcn1 突变被转移 DNA (T-DNA) 标记，并克隆了 T-DNA 插入片段侧翼的序列，对 RCN1 cDNA 的分析表明，T-DNA 插入破坏了蛋白磷酸酶 2A (PP2A-A) 调节 A 亚基的基因，RCN1 基因挽救了 rcn1 突变体表型，并且还补充了酿酒酵母 PP2A-A 的温度敏感表型突变，tpd3-1，这些数据暗示蛋白磷酸酶 2A ire 对拟南芥中生长素运输的调节。
The phytohormone auxin controls processes such as cell elongation, root hair development and root branching, Tropisms, growth curvatures triggered by gravity, light and touch, are also auxin-mediated responses, Auxin is synthesized in the shoot apex and transported through the stem, but the molecular mechanism of auxin transport is not well understood, Naphthylphthalamic acid (NPA) and other inhibitors of auxin transport block tropic curvature responses and inhibit root and shoot elongation, We have isolated a novel Arabidopsis thaliana mutant designated roots curl in NPA (rcn1), Mutant seedlings exhibit altered responses to NPA in root curling and hypocotyl elongation, Auxin efflux in mutant seedlings displays increased sensitivity to NPA. The rcn1 mutation was transferred-DNA (T-DNA) tagged and sequences flanking the T-DNA insert were cloned, Analysis of the RCN1 cDNA reveals that the T-DNA insertion disrupts a gene for the regulatory A subunit of protein phosphatase 2A (PP2A-A), The RCN1 gene rescues the rcn1 mutant phenotype and also complements the temperature-sensitive phenotype of the Saccharomyces cerevisiae PP2A-A mutation, tpd3-1, These data implicate protein phosphatase 2A ire the regulation of auxin transport in Arabidopsis.