In vivo neurogenesis in the dorsal vagal complex of the adult rat brainstem
In vivo neurogenesis in the dorsal vagal complex of the adult rat brainstem
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DOI：
10.1016/j.neuroscience.2004.08.047
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Moyse, E
中科院分区：
文献类型：
--
作者：
Bauer, S;Hay, M;Moyse, E
迷走神经背侧复合体（dorsal vagal complex，DVC）由孤束核（nucleus tractus solitarii，NTS）、迷走神经背侧运动核（dorsal motor nucleus of vagus nerve，DMX）和最后区（area postrema，AP）组成，是哺乳动物自主神经系统的主要整合中枢。据报道，成年大鼠DVC含有依赖于传入神经的神经细胞粘附分子的可塑性促进聚唾液酸化形式的浓度[J Neurosci 21（2001）4721; Eur J Neurosci 14（2001）1194]。这促使我们评估成年大鼠DVC中神经发生的发生。在整个DVC范围内，将腹膜内溴脱氧尿苷（BrdU）注射液对细胞增殖的累积体内标记与表型标记物和连续脑干切片上的共聚焦显微镜检查相结合。在基础条件下，稀疏的BrdU+细胞核中选择性地检测到DVC根据离散和可重复的模式。发现其中一些与神经元标记物doublecortin、HuC/D或神经元特异性抗原（NeuN）共定位，表明神经发生确实发生在成年大鼠的DVC内。在NTS中，10%的BrdU+细胞核也是NeuN+。在DVC中发现了相当比例的星形胶质细胞生成。巢蛋白免疫组化在AP和NTS之间的边界产生了高度特异性的标记模式。这些数据可能与第四脑室底部的神经干细胞有关[J Neurosci 16（1996）7599]。为了评估体内传入输入对神经发生的可能调节，在累积BrdU治疗之前进行单侧迷走神经切断术。这种DVC去传入触发了同侧DVC中BrdU掺入的大量增加，这与DIVIX中的小胶质细胞增殖以及NTS中神经元和星形胶质细胞的生成增加有关。这些发现建立DVC作为一种新的模型，成人神经发生是反应性的去传入。(C)2004年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The dorsal vagal complex (DVC) encompasses the nucleus tractus solitarii (NTS), the dorsal motor nucleus of the vagus nerve (DMX) and the area postrema (AP), that altogether provide the major integrative center for the mammalian autonomic nervous system. The adult rat DVC has been reported to contain afferent-dependent concentration of the plasticity-promoting polysialylated form of neural cell adhesion molecule [J Neurosci 21 (2001) 4721; Eur J Neurosci 14 (2001) 1194]. This prompted us to assess the occurrence of neurogenesis in the DVC of adult rats. Cumulative in vivo labeling of cell proliferation with i.p. bromodeoxyuridine (BrdU) injections was combined with phenotypic markers and confocal microscopy on serial brainstem sections throughout the DVC extent. In basal condition, sparse BrdU+ nuclei were selectively detected in the DVC according to a discrete and reproducible pattern. Some of them were found to colocalize with the neuronal markers doublecortin, HuC/D, or neuronal-specific antigen (NeuN), demonstrating that neurogenesis does occur within the DVC of adult rat. In the NTS, 10% of the BrdU+ nuclei were also NeuN+. A comparable proportion of astrogliogenesis was found in the DVC. Nestin immunohistochemistry yielded a highly specific labeling pattern at the border between AP and NTS. These data may relate to the neural stem cells that have been reported in the floor of the IVth ventricle [J Neurosci 16 (1996) 7599]. In order to assess a possible modulation of neurogenesis by afferent input in vivo, unilateral vagotomy was performed prior to cumulative BrdU treatment. Such DVC deafferentation triggered a large increase of BrdU incorporation in the ipsilateral DVC, which was associated with microglial proliferation in the DIVIX and with increased genesis of neurons and astrocytes in the NTS. These findings establish DVC as a novel model of adult neurogenesis that is reactive to deafferentation. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.