Oocyte CTR1 is not essential for cisplatin-induced oocyte death of primordial follicle.
Oocyte CTR1 is not essential for cisplatin-induced oocyte death of primordial follicle.
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DOI：
10.17912/micropub.biology.000632
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发表时间：
2022
影响因子：
--
通讯作者：
Kim, So-Youn
中科院分区：
文献类型：
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作者：
Yu, Seok-Yeong;Luan, Yi;Abazarikia, Amirhossein;Dong, Rosemary;Lee, Jaekwon;Kim, So-Youn
累积的证据表明，顺铂，一种铂基烷化剂，导致优先DNA损伤的卵母细胞的原始卵泡（PF）在卵巢中，表明卵母细胞的顺铂的青睐积累。铜转运蛋白1（CTR 1; Slc 31 a1）参与促进顺铂在细胞中的摄取。我们发现在小鼠卵巢组织中，PFs卵母细胞中CTR 1的表达高于其他类型的细胞。然而，卵母细胞特异性Slc 31 a1基因敲除不足以防止顺铂诱导的体外PFs耗竭。我们的数据表明，CTR 1不会是顺铂在卵巢中PF卵母细胞内转运的唯一途径。
Accumulated evidence indicates that cisplatin, a platinum-based alkylating agent, causes preferential DNA damage to oocytes of primordial follicles (PFs) in the ovary, suggesting oocyte-favored accumulation of cisplatin. Copper transporter 1 (CTR1; Slc31a1 ) is implicated in facilitating cisplatin uptake in cells. Here we found that oocytes of PFs had constitutively higher expression of CTR1 than other cell types in mouse ovary. However, oocyte-specific Slc31a1 knockout was not sufficient to prevent cisplatin-induced depletion of PFs in vitro . Our data indicate that CTR1 would not be the only route for cisplatin to be transported inside the oocytes of PFs in the ovary.