Synthesis of D-Mannose Capped Silicon Nanoparticles and Their Interactions with MCF-7 Human Breast Cancerous Cells
Synthesis of D-Mannose Capped Silicon Nanoparticles and Their Interactions with MCF-7 Human Breast Cancerous Cells
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DOI：
10.1021/am4017126
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发表时间：
2013-08-14
影响因子：
9.5
通讯作者：
Chao, Yimin
中科院分区：
文献类型：
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作者：
Ahire, Jayshree H.;Chambrier, Isabelle;Chao, Yimin
硅纳米颗粒（SiNPs）在生物医学应用的各个方面都有着突出的兴趣。为此，纳米颗粒的表面功能化对于稳定它们、将它们靶向特定疾病区域并使它们选择性地结合细胞或细胞表面上存在的生物分子至关重要。然而，没有这样的官能化已经探索与硅纳米粒子。碳水化合物在细胞识别中起着关键作用。在这里，我们报告的第一个合成的硅纳米粒子与碳水化合物功能化。在这项研究中，稳定和明亮的发光D-甘露糖（Man）封端的SiNPs已经从胺封端的SiNPs和D-吡喃甘露糖苷酸合成。通过傅里叶变换红外光谱（FTIR）、核磁共振光谱（NMR）和能量色散X射线光谱（EDX）研究证实了表面官能化。晶体核心的平均直径为5.5 nm，通过透射电子显微镜（TEM）测量，而通过动态光散射（DLS）获得的流体动力学直径为16 nm。光致发光发射的量子产率（QY）被发现是11.5%，和纳米粒子表现出卓越的稳定性超过两个星期。Man-capped SiNPs可能被证明是进一步研究糖生物学、生物医学和材料科学领域的有价值的工具。为了证明这一假设，使用伴刀豆球蛋白A（ConA）作为靶蛋白进行了实验。当Man官能化的SiNP用ConA处理时，形成交联的聚集体，如TEM图像所示以及通过光致发光光谱（PL）监测。Man官能化的SiNP可以靶向癌细胞。MCF-7人乳腺癌细胞中SiNP的可视化成像显示荧光分布在这些细胞的整个细胞质中。
Silicon nanoparticles (SiNPs) hold prominent interest in various aspects of biomedical applications. For this purpose, surface functionalization of the NPs is essential to stabilize them, target them to specific disease area, and allow them to selectively bind to the cells or the bio-molecules present on the surface of the cells. However, no such functionalization has been explored with Si nanoparticles. Carbohydrates play a critical role in cell recognition. Here, we report the first synthesis of silicon nanoparticles functionalized with carbohydrates. In this study, stable and brightly luminescent D-Mannose (Man) capped SiNPs have been synthesized from amine terminated SiNPs and D-mannopyranoside acid. The surface functionalization is confirmed by Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), and energy dispersive X-ray spectroscopy (EDX) studies. The mean diameter of the crystal core is 5.5 nm, as measured by transmission electron microscopy (TEM), while the hydrodynamic diameter obtained by dynamic light scattering (DLS) is 16 nm. The quantum yield (QY) of photoluminescence emission is found to be 11.5%, and the nanoparticles exhibit an exceptional stability over two weeks. The Man-capped SiNPs may prove to be valuable tools for further investigating glycobiological, biomedical, and material science fields. Experiments are carried out using Concanavalin A (ConA) as a target protein in order to prove the hypothesis. When Man functionalized SiNPs are treated with ConA, cross-linked aggregates are formed, as shown in TEM images as well as monitored by photoluminescence spectroscopy (PL). Man functionalized SiNPs can target cancerous cells. Visualization imaging of SiNPs in MCF-7 human breast cancer cells shows the fluorescence is distributed throughout the cytoplasm of these cells.