Metabolism of nitrilotriacetate by cells of Pseudomonas species.
Metabolism of nitrilotriacetate by cells of Pseudomonas species.
复制标题
假单胞菌属细胞对次氮基三乙酸的代谢。
DOI：
10.1128/aem.25.5.811-818.1973
复制
发表时间：
1973
期刊：
影响因子：
--
通讯作者：
E. J. Malec
中科院分区：
文献类型：
--
作者：
J. Tiedje;B. Mason;C. Warren;E. J. Malec
从土壤中分离出一种假单胞菌，无论存在或不存在几种抑制剂，该假单胞菌都可以将次氮基三乙酸 (NTA) 降解为 CO(2)、H(2)O、NH(3) 和细胞成分，而不会积累大量中间体。经过广泛的气相色谱分析，少量的天冬氨酸、甘氨酸和乌头酸是 NTA 降解过程中唯一可检测到的积累化合物，并且这些化合物不会从细胞中排出。测压研究表明，亚氨基二乙酸盐、甘氨酸和乙醛酸盐是可能的中间体，而 N-甲基亚氨基二乙酸盐、肌氨酸和乙酸盐则不是。该数据与 NTA C-N 键的氧化裂解作为初始降解步骤一致。
A Pseudomonas species was isolated from soil which could degrade nitrilotriacetate (NTA) to CO(2), H(2)O, NH(3), and cellular constituents without the accumulation of significant quantities of intermediates either in the presence or absence of several inhibitors. After extensive gas chromatography analysis, small quantities of aspartate, glycine, and aconitate were the only detectable compounds to accumulate during NTA degradation, and these compounds were not excreted from the cells. Manometric studies indicated that iminodiacetate, glycine, and glyoxylate are possible intermediates, whereas N-methyliminodiacetate, sarcosine, and acetate are not. The data are consistent with an oxidative cleavage of the C-N bond of NTA as the initial degradation step.