Highly sensitive detection of GG mismatched DNA by surfaces immobilized naphthyridine dimer through poly(ethylene oxide) linkers.
Highly sensitive detection of GG mismatched DNA by surfaces immobilized naphthyridine dimer through poly(ethylene oxide) linkers.
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DOI：
10.1016/j.bmcl.2003.12.079
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发表时间：
2004-03
影响因子：
2.7
通讯作者：
K. Nakatani;A. Kobori;H. Kumasawa;I. Saito
中科院分区：
文献类型：
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作者：
K. Nakatani;A. Kobori;H. Kumasawa;I. Saito
萘二聚体是一种独特的分子，可以选择性地与双链DNA中的鸟嘌呤-鸟嘌呤错配结合。我们合成了具有不同长度的聚环氧乙烷（PEO）连接体的萘二聚体，并将其固定在CM5传感器芯片上，对含有单碱基错配的DNA双链进行了表面等离子体共振（SPR）检测。传感器的灵敏度随着加入到连接器中的PEO单元数量的增加而显著增加。通过三个PEO单元，将传感器表面固定为1.5×103response单元（RU）的萘二聚体，在27-mer G-G错配浓度为1 nM时，观察到明显的SPR信号。
Naphthyridine dimer is a unique molecule that strongly, and selectively, binds to the guanine–guanine mismatch in duplex DNA. We have synthesized naphthyridine dimers possessing a different length of poly(ethylene oxide) (PEO) linker, and immobilized them to CM5 sensor chip to carry out a surface plasmon resonance (SPR) assay of DNA duplexes containing a single base mismatch. The sensitivity of the sensor remarkably increased with increasing numbers of PEO units incorporated into the linker. With the sensor surface immobilized naphthyridine dimer for 1.5×103response unit (RU) through three PEO units, the distinct SPR signal was observed at a concentration of 1 nM of the 27-mer G–G mismatch.