MRP functional activity and character in the dinoflagellate Prorocentrum lima
MRP functional activity and character in the dinoflagellate Prorocentrum lima
复制标题
甲藻原甲藻中 MRP 功能活性和特征
DOI：
10.1007/s10811-015-0679-1
复制
发表时间：
2016-06-01
影响因子：
3.3
通讯作者：
Yang, Wei-dong
中科院分区：
文献类型：
--
作者：
Hou, Dao-yi;Liang, Jin-jin;Yang, Wei-dong
利用激光扫描共聚焦显微镜（LACM）和流式细胞仪（FCM）研究发现，多药耐药相关蛋白（MRP）特异性抑制剂MK 571可增加产DSP毒素的利马原甲藻（Prorocentrum lima）中钙黄绿素-AM的积累。这导致了这样的假设，即利马青霉的细胞在质膜上具有MRP转运蛋白。在培养后4、20、28、42 d和38 d的限制氮、磷条件下，测定了利玛原甲藻DSP毒素的主要成分冈田酸（OA）含量和MRP活性。我们发现，无论是OA水平和MRP活性在利马的增加随着时间的推移，OA浓度和MRP活性一致增加，与对照组相比，在N-和P-限制条件下。OA浓度的这些变化和相关的MRP活性的增加表明MRP可能参与了P. lima中OA的转运。然而，MK 571和CsA（一种多药耐药（MDR）相关ABC蛋白的广谱抑制剂）对P. lima中OA含量无明显影响。除MDR相关的ABC蛋白外，其他转运蛋白如PDR可能参与了OA在利马毕赤酵母中的转运。
Using laser scanning confocal microscope (LACM) and flow cytometry (FCM), we found that MK571, a specific inhibitor of multidrug resistance-associated protein (MRP), could increase the accumulation of calcein-AM in Prorocentrum lima, a DSP toxin-producing dinoflagellate. This led to the assumption that the cells of P. lima possess MRP transporters in the plasma membrane. The contents of okadaic acid (OA), a main component of DSP toxins, and MRP activities in P. lima were determined at 4, 20, 28, and 42 days after culture and at 38 day under N- and P-limited conditions. We found that both the OA level and MRP activity in P. lima increased over time; OA concentration and MRP activity conformably increased under N- and P-limited conditions compared with control counterparts. These changes in OA concentration and associated increased MRP activity suggest that MRP might be involved in OA transport in P. lima. However, MK571 and CsA, a broad-spectrum inhibitor of multidrug resistance (MDR)-associated ABC proteins, had no observable effects on OA content in P. lima. It seems that except for MDR-associated ABC proteins, other transporters like PDR might be involved in the transport of OA in P. lima.