Bilirubin oxidase from Bacillus pumilus: a promising enzyme for the elaboration of efficient cathodes in biofuel cells.
Bilirubin oxidase from Bacillus pumilus: a promising enzyme for the elaboration of efficient cathodes in biofuel cells.
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DOI：
10.1016/j.bios.2012.02.033
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发表时间：
2012-05-15
影响因子：
12.6
通讯作者：
Mano, Nicolas
中科院分区：
文献类型：
--
作者：
Durand, Fabien;Kjaergaard, Christian Hauge;Suraniti, Emmanuel;Gounel, Sebastien;Hadt, Ryan G.;Solomon, Edward I.;Mano, Nicolas
从短小芽孢杆菌（Bacillus pumilus）中分离到一种科塔多铜氧化酶（MCO），它是一种新的细菌胆红素氧化酶（BOD）。在大肠杆菌中成功地过量表达了含有四个铜的59kDa蛋白，并一步纯化至均一。该509个氨基酸的酶，分别与来自枯草芽孢杆菌的科塔和来自疣状漆斑菌的BOD具有67%和26%的序列同一性，与其他细菌MCO相比，对胆红素显示出更高的周转活性。当固定在氧化还原水凝胶中时，用于O2还原的电流密度仅比用松毛虫BOD获得的电流小12%。在连续电催化条件下，新BOD修饰电极比T.松藻BOD修饰电极这使得BOD来自B。pumilus在生物燃料电池和生物传感器中的应用是一个有吸引力的新候选者。
A CotA Multicopper Oxidase (MCO) from Bacillus pumilus, previously identified as a laccase, has been studied and characterized as a new bacterial Bilirubin Oxidase (BOD). The 59kDa protein containing four coppers, was successfully over-expressed in Escherichia coli and purified to homogeneity in one step. This 509 amino-acid enzyme, having 67% and 26% sequence identity with CotA from Bacillus subtilis and BOD from Myrothecium verrucaria, respectively, shows higher turnover activity towards bilirubin compared to other bacterial MCOs. The current density for O2 reduction, when immobilized in a redox hydrogel, is only 12% smaller than the current obtained with Trachyderma tsunodae BOD. Under continuous electrocatalysis, an electrode modified with the new BOD is more stable, and has a higher tolerance towards NaCl, than a T. tsunodae BOD modified electrode. This makes BOD from B. pumilus an attractive new candidate for application in biofuel cells and biosensors.
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DOI：
10.1039/c1cc15002b
发表时间：
2011-11-21
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Rengaraj S;Mani V;Kavanagh P;Rusling J;Leech D
通讯作者：
Leech D
影响因子：
12.6
作者：
Gallaway, Joshua;Wheeldon, Ian;Barton, Scott Calabrese
通讯作者：
Barton, Scott Calabrese
影响因子：
4.9
作者：
Mano, Nicolas
通讯作者：
Mano, Nicolas
影响因子：
2.8
作者：
Pakhadnia, Y. G.;Malinouski, N. I.;Lapko, A. G.
通讯作者：
Lapko, A. G.
影响因子：
4.3
作者：
Ramirez, Pablo;Mano, Nicolas;Shleev, Sergey
通讯作者：
Shleev, Sergey