A Magnetic Iron Oxide/Polydopamine Coating Can Improve Osteogenesis of 3D-Printed Porous Titanium Scaffolds with a Static Magnetic Field by Upregulating the TGFβ-Smads Pathway
A Magnetic Iron Oxide/Polydopamine Coating Can Improve Osteogenesis of 3D-Printed Porous Titanium Scaffolds with a Static Magnetic Field by Upregulating the TGFβ-Smads Pathway
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DOI：
10.1002/adhm.202000318
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发表时间：
2020-06-16
影响因子：
10
通讯作者：
Qiu, Guixing
中科院分区：
文献类型：
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作者：
Huang, Zhenfei;He, Yu;Qiu, Guixing
3D 打印多孔钛铝钒 (Ti6Al4V, pTi) 支架为外科医生提供了重建大骨缺损的良好选择，尤其是在承重部位。但成骨能力差限制了其临床应用。本研究通过在3D打印的pTi支架表面共沉积Fe(3)O(4)纳米粒子和聚多巴胺(PDA)成功制备了一种新型磁性涂层，该涂层在体外增强hBMSC的细胞附着、增殖和成骨分化，以及在有/无静磁场(SMF)的情况下体内兔股骨骨缺损的新骨形成。此外，通过蛋白质组学分析，发现磁性 Fe3O4/PDA 涂层与 SMF 增强的成骨作用与上调 TGF beta-Smads 信号通路有关。因此，这项工作提供了一个简单的方案来改善3D打印多孔pTi支架的成骨作用，这将有助于其在临床中的应用。
3D-printed porous titanium-aluminum-vanadium (Ti6Al4V, pTi) scaffolds offer surgeons a good option for the reconstruction of large bone defects, especially at the load-bearing sites. However, poor osteogenesis limits its application in clinic. In this study, a new magnetic coating is successfully fabricated by codepositing of Fe(3)O(4)nanoparticles and polydopamine (PDA) on the surface of 3D-printed pTi scaffolds, which enhances cell attachment, proliferation, and osteogenic differentiation of hBMSCs in vitro and new bone formation of rabbit femoral bone defects in vivo with/without a static magnetic field (SMF). Furthermore, through proteomic analysis, the enhanced osteogenic effect of the magnetic Fe3O4/PDA coating with the SMF is found to be related to upregulate the TGF beta-Smads signaling pathway. Therefore, this work provides a simple protocol to improve the osteogenesis of 3D-printed porous pTi scaffolds, which will help their application in clinic.