Activated platelets express IL-1 activity.
Activated platelets express IL-1 activity.
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DOI：
10.4049/jimmunol.143.12.4015
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发表时间：
1989-12
影响因子：
4.4
通讯作者：
C. Hawrylowicz;S. Santoro;F. Platt;E. Unanue
中科院分区：
文献类型：
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作者：
C. Hawrylowicz;S. Santoro;F. Platt;E. Unanue
经洗涤的人血小板的悬浮液在用诸如凝血酶、胶原蛋白、ADP或肾上腺素的试剂活化后表达IL-1活性，这是通过血小板悬浮液支持T细胞系D10.G4.1生长的能力来判断的，所述T细胞系D10.G4.1表现出对IL-1的生长需求。未活化的血小板表达很少的IL-1活性。活化血小板表达的IL-1活性似乎完全与血小板表面相关。在来自活化血小板悬浮液的上清液中未检测到IL-1活性。IL-1 β特异性mAb可抑制凝血酶活化血小板表达的90%活性，而IL-1 α特异性mAb可抑制约20%活性。对照mAb没有效果。这些结果表明，活化的血小板表达表面相关的IL-1活性。血小板表面IL-1可能提供一种机制，以极其局部和快速的方式改变IL-1反应细胞的性质，在炎症和血管壁损伤过程中血小板与IL-1反应细胞直接接触。
Suspensions of washed human platelets express IL-1 activity after activation with agents such as thrombin, collagen, ADP, or epinephrine as judged by the ability of the platelet suspensions to support the growth of a T cell line, D10.G4.1, which exhibits a growth requirement for IL-1. Unactivated platelets express little IL-1 activity. The IL-1 activity expressed by activated platelets appears to be entirely associated with the platelet surface. No IL-1 activity was detected in supernatants derived from suspensions of activated platelets. A mAb specific for IL-1 beta inhibited 90% of the activity expressed by thrombin-activated platelets, whereas a mAb specific for IL-1 alpha inhibited approximately 20% of the activity. A control mAb was without an effect. These results indicate that activated platelets express surface-associated IL-1 activity. Platelet surface IL-1 may provide a mechanism for altering in an extremely localized and rapid manner the properties of IL-1 responsive cells with which platelets come in direct contact during processes of inflammation and vessel wall damage.