A putative Arabidopsis nucleoporin, AtNUP160, is critical for RNA export and required for plant tolerance to cold stress
A putative Arabidopsis nucleoporin, AtNUP160, is critical for RNA export and required for plant tolerance to cold stress
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DOI：
10.1128/mcb.01063-06
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发表时间：
2006-12-01
影响因子：
5.3
通讯作者：
Zhu, Jian-Kang
中科院分区：
文献类型：
--
作者：
Dong, Chun-Hai;Hu, Xiangyang;Zhu, Jian-Kang
为了研究植物对冷胁迫反应的遗传控制，通过筛选损害CBF3-Luc报告基因转录的突变来分离拟南芥突变体。我们在这里报道了一个突变基因atnup160-1的特征和克隆，该突变基因在冷胁迫下导致CBF3-Luc诱导降低。Atnup160-1突变植株表现出冷响应基因表达的改变，对低温胁迫敏感，获得的耐寒性存在缺陷。AtNUP160是通过定位克隆获得的，编码动物核孔蛋白Nup160的同源物。微阵列分析表明，除了CBF基因的表达受损外，突变体中还影响到其他一些与植物耐冷性有关的基因。Atnup160突变体开花早，幼苗生长迟缓，特别是在低温条件下。AtNUP160蛋白定位于核缘，Poly(A)-mRNA原位杂交结果表明，在atnup160-1突变植株中，其mRNA输出存在缺陷。我们的研究表明，拟南芥AtNUP160是核质运输的关键基因，它在植物生长和开花时间调控中起着重要作用，是耐冷性所必需的。
To study the genetic control of plant responses to cold stress, Arabidopsis thaliana mutants were isolated by a screen for mutations that impair cold-induced transcription of the CBF3-LUC reporter gene. We report here the characterization and cloning of a mutated gene, atnup160-1, which causes reduced CBF3-LUC induction under cold stress. atnup160-1 mutant plants display altered cold-responsive gene expression and are sensitive to chilling stress and defective in acquired freezing tolerance. AtNUP160 was isolated through positional cloning and shown to encode a putative homolog of the animal nucleoporin Nup160. In addition to the impaired expression of CBF genes, microarray analysis revealed that a number of other genes important for plant cold tolerance were also affected in the mutants. The atnup160 mutants flower early and show retarded seedling growth, especially at low temperatures. AtNUP160 protein is localized at the nuclear rim, and poly(A)-mRNA in situ hybridization shows that mRNA export is defective in the atnup160-1 mutant plants. Our study suggests that Arabidopsis AtNUP160 is critical for the nucleocytoplasmic transport of mRNAs and that it plays important roles in plant growth and flowering time regulation and is required for cold stress tolerance.