Microbial Enterotypes, Inferred by the Prevotella-to-Bacteroides Ratio, Remained Stable during a 6-Month Randomized Controlled Diet Intervention with the New Nordic Diet
Microbial Enterotypes, Inferred by the Prevotella-to-Bacteroides Ratio, Remained Stable during a 6-Month Randomized Controlled Diet Intervention with the New Nordic Diet
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DOI：
10.1128/aem.03549-13
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发表时间：
2014-02-01
影响因子：
4.4
通讯作者：
Bahl, Martin I.
中科院分区：
文献类型：
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作者：
Roager, Henrik M.;Licht, Tine R.;Bahl, Martin I.
有人认为，人类肠道微生物群可以根据特定细菌群的丰度分为肠型;然而，这些肠型的生物学意义和稳定性仍未得到解决。在这里，我们证明，受试者（n = 62）18至65岁的中心性肥胖和代谢综合征的组成部分，可以分为两个离散的组，简单地通过他们的相对丰富的普雷沃氏菌属。分类：Bacteroides spp.（P/B比率）。此外，我们表明，这些组在6个月的受控饮食干预期间保持稳定，其中研究了与新北欧饮食（NND）建议一致的饮食（雅阁）与平均丹麦饮食（ADD）对肠道微生物群的影响。在这项研究中，受试者（根据P/B比分层和不分层）通过定量PCR（ADD与NND相比）定量的35种选定细菌分类群未显示出饮食干预导致的显著变化。然而，我们发现干预后高P/B组的血浆总胆固醇高于低P/B组。我们提出，简单地根据P/B比值对人类进行分层，可以更好地评估干预措施对肠道微生物群和生理生物标志物的可能影响。
It has been suggested that the human gut microbiota can be divided into enterotypes based on the abundance of specific bacterial groups; however, the biological significance and stability of these enterotypes remain unresolved. Here, we demonstrated that subjects (n = 62) 18 to 65 years old with central obesity and components of metabolic syndrome could be grouped into two discrete groups simply by their relative abundance of Prevotella spp. divided by Bacteroides spp. (P/B ratio) obtained by quantitative PCR analysis. Furthermore, we showed that these groups remained stable during a 6-month, controlled dietary intervention, where the effect of consuming a diet in accord with the new Nordic diet (NND) recommendations as opposed to consuming the average Danish diet (ADD) on the gut microbiota was investigated. In this study, subjects (with and without stratification according to P/B ratio) did not reveal significant changes in 35 selected bacterial taxa quantified by quantitative PCR (ADD compared to NND) resulting from the dietary interventions. However, we found higher total plasma cholesterol within the high-P/B group than in the low-P/B group after the intervention. We propose that stratification of humans based simply on their P/B ratio could allow better assessment of possible effects of interventions on the gut microbiota and physiological biomarkers.