The effect of glutamate on ghrelin release in mice.
The effect of glutamate on ghrelin release in mice.
复制标题
谷氨酸对小鼠胃饥饿素释放的影响。
DOI：
10.1002/cbin.10728
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发表时间：
2017
影响因子：
3.9
通讯作者：
Ichiro Sakata
中科院分区：
文献类型：
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作者：
Rakhi Chacrabati;Zhi Gong;Chika Ikenoya;Daisuke Kondo;Jeffrey M. Zigman;Takafumi Sakai;Ichiro Sakata
胃饥饿素在胃中大量产生。在这里，我们发现谷氨酸降低了生长素释放肽产生细胞中的生长素释放肽表达和释放，并降低了小鼠的食物摄入量和血浆酰基生长素释放肽水平。在生长素释放肽产生细胞系中用G蛋白偶联受体家族C组5成员B（GPRC 5 B）的siRNA处理完全阻断谷氨酸诱导的生长素释放肽抑制作用。此外，谷氨酸通过细胞外信号调节激酶（ERK）活性途径抑制ghrelin释放，并刺激产生ghrelin的细胞系中CREB 2 mRNA的表达。这些结果表明，谷氨酸通过ERK-CREB 2途径抑制ghrelin释放。这些结果表明，GPRC 5 B-ERK-CREB 2通路参与了ghrelin细胞中ghrelin表达和分泌的抑制。
Ghrelin is abundantly produced in the stomach. Here, we found that glutamate decreased ghrelin expression and release in ghrelin‐producing cells, and decreased levels of food intake and plasma acyl‐ghrelin in mice. Treatment with siRNA of G protein‐coupled receptor, family C, group 5, member B (GPRC5B) in ghrelin‐producing cell lines completely blocked the effect of glutamate‐induced ghrelin suppression. In addition, glutamate inhibited ghrelin release via the extracellular signal‐regulated kinase (ERK) activity pathway, and stimulated CREB2 mRNA expression in ghrelin‐producing cell lines. These results suggest that glutamate inhibits ghrelin release via ERK‐CREB2 pathway. These results suggest that the GPRC5B‐ERK‐CREB2 pathway is involved in the inhibition of ghrelin expression and secretion in ghrelin cells.