Three representative inter and intra-subspecific crosses reveal the genetic architecture of reproductive isolation in rice
Three representative inter and intra-subspecific crosses reveal the genetic architecture of reproductive isolation in rice
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三个代表性的亚种间和亚种内杂交揭示了水稻生殖隔离的遗传结构
DOI：
10.1111/tpj.13661
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发表时间：
2017-11-01
期刊：
影响因子：
7.2
通讯作者：
Ouyang, Yidan
中科院分区：
文献类型：
--
作者：
Li, Guangwei;Li, Xiaoting;Ouyang, Yidan
系统研究杂种不育性形成的遗传和分子机制对于理解生殖隔离和物种形成具有重要意义。利用超高密度遗传图谱，在3个代表性粳稻品种与2个籼稻品种组的杂交组合中，共检测到43个单基因QTL和223个双基因互作，它们与胚囊、花粉和小穗育性有关，为研究水稻生殖隔离的遗传基础提供了丰富的资料。鉴定了10个新检测到的亚种间和亚种内育性单位点QTL。在NipxMH 63和NipxMH 97两个组合中都检测到3个控制胚囊育性的基因座，而控制花粉育性的QTL在两个组合中不相同，从而导致育性变异。在1997 × MH 63中发现了5个与育性和分离畸变有关的基因座。在全基因组中量化了两位点相互作用对育性的重要性，确定了三种类型的相互作用有助于杂种育性降低。这些结果构建了水稻不同形式生殖障碍的遗传结构，对于利用亚种间杂种优势沿着提高籼-籼杂种的单位点和多位点育性具有重要意义。
Systematic characterization of genetic and molecular mechanisms in the formation of hybrid sterility is of fundamental importance in understanding reproductive isolation and speciation. Using ultra-high-density genetic maps, 43 single-locus quantitative trait loci (QTLs) and 223 digenic interactions for embryo-sac, pollen, and spikelet fertility are depicted from three crosses between representative varieties of japonica and two varietal groups of indica, which provide an extensive archive for investigating the genetic basis of reproductive isolation in rice. Ten newly detected single-locus QTLs for inter- and intra-subspecific fertility are identified. Three loci for embryo-sac fertility are detected in both NipxZS97 and NipxMH63 crosses, whereas QTLs for pollen fertility are not in common between the two crosses thus leading to fertility variation. Five loci responsible for fertility and segregation distortion are observed in the ZS97xMH63 cross. The importance of two-locus interactions on fertility are quantified in the whole genome, which identify that three types of interaction contribute to fertility reduction in the hybrid. These results construct the genetic architecture with respect to various forms of reproductive barriers in rice, which have significant implications in utilization of inter-subspecific heterosis along with improvement in the fertility of indica-indica hybrids at single- and multi-locus level.