Simple autofocusing method by image processing using transmission images for large-scale two-photon lithography
Simple autofocusing method by image processing using transmission images for large-scale two-photon lithography
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DOI:
10.1364/oe.390486
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发表时间:
2020-04-13
期刊:
影响因子:
3.8
通讯作者:
Maruo, Shoji
中科院分区:
文献类型:
--
作者:
Fujishiro, Yoko;Furukawa, Taichi;Maruo, Shoji
We propose a simple autofocusing technique that can be introduced into conventional two-photon lithography systems without additional devices. Autofocusing is achieved by image processing using transmission images of photopolymerized voxels. The signal-to-noise ratio of transmission images was improved by optimal low-pass filtering to detect voxels in them. The focal point was detected with an accuracy of about 250 nm from the difference images. Further, we demonstrated mass-fabrication of a 5 x 5 spiral square array with an area of 900 x 900 mu m(2) using this method. The method has potential application in constructing low-cost, compact and versatile two-photon lithography apparatus. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement