Methanol extract of Sanguisorba officinalis L. with cytotoxic activity against PC3 human prostate cancer cells
Methanol extract of Sanguisorba officinalis L. with cytotoxic activity against PC3 human prostate cancer cells
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DOI：
10.3892/mmr.2012.949
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发表时间：
2012-09-01
影响因子：
3.4
通讯作者：
Cho, Sung-Dae
中科院分区：
文献类型：
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作者：
Choi, Eun-Sun;Kim, Jun-Sung;Cho, Sung-Dae
地榆是一种天然植物，传统上用于治疗炎症和代谢性疾病。多项研究报告称，其提取物具有抗癌、抗氧化和抗脂质过氧化活性。然而，这种植物对人类前列腺癌细胞的影响尚未得到研究。在本研究中，我们研究了地榆甲醇提取物（MESO）对 PC3 人前列腺癌细胞的抑制作用和潜在机制。 MESO 通过内在凋亡途径显着降低细胞生长并诱导细胞凋亡。 MESO 降低了骨髓细胞白血病-1 (Mcl-1) 的表达水平，Mcl-1 是一种 Bcl-2 样抗凋亡蛋白，在多种癌细胞系中高度表达。 MESO 增加了促凋亡蛋白 Bax 的表达水平，而 Bak 和 Bcl-xL 的表达水平没有变化。此外，MESO 诱导线粒体外膜中 Bax 的寡聚化。这些结果表明，MESO 通过下调 Mcl-1 蛋白表达和 Bax 寡聚化来抑制前列腺癌细胞的生长并诱导细胞凋亡。因此，MESO有潜力作为治疗前列腺癌的候选药物。
Sanguisorba officinalis is a natural plant that has been traditionally used for the treatment of inflammatory and metabolic diseases. Several studies have reported that its extracts exhibit anticancer, antioxidative and anti-lipid peroxidation activities. However, the effects of this plant on human prostate cancer cells have not yet been investigated. In the present study, we investigated the inhibitory effects and underlying mechanisms of a methanol extract of Sanguisorba officinalis (MESO) in PC3 human prostate cancer cells. MESO significantly decreased cell growth and induced apoptosis through the intrinsic apoptosis pathway. MESO decreased the expression levels of myeloid cell leukemia-1 (Mcl-1), a Bcl-2-like anti-apoptotic protein that is highly expressed in various cancer cell lines. Expression levels of the pro-apoptotic protein Bax were increased by MESO whereas those of Bak and Bcl-xL were unchanged. In addition, MESO induced the oligomerization of Bax in the mitochondrial outer membrane. These results suggest that MESO inhibits the growth of prostate cancer cells and induces apoptotic cell death by the downregulation of Mcl-1 protein expression and the oligomerization of Bax. Therefore, MESO has potential as a drug candidate for the treatment of prostate cancer.