APOPTOTIC CELL-DEATH IN HUMAN GASTRIC-CARCINOMA - ANALYSIS BY TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE-MEDIATED DUTP-BIOTIN NICK END LABELING
APOPTOTIC CELL-DEATH IN HUMAN GASTRIC-CARCINOMA - ANALYSIS BY TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE-MEDIATED DUTP-BIOTIN NICK END LABELING
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DOI：
10.1111/j.1349-7006.1994.tb02972.x
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发表时间：
1994-09-01
期刊：
影响因子：
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通讯作者：
ITO, H
中科院分区：
文献类型：
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作者：
KASAGI, N;GOMYO, Y;ITO, H
我们研究了发生凋亡细胞死亡的福尔马林固定，石蜡包埋的人胃癌标本的末端脱氧核苷酸转移酶（TdT）介导的dUTP-生物素缺口末端标记（TUNEL）方法。TUNEL信号的特异性通过省略TdT或生物素化dUTP作为阴性对照以及通过用DNA酶I预处理作为阳性对照来证实。仔细观察常规苏木精和伊红染色切片显示少数肿瘤细胞凋亡，尤其是在高分化癌中。即使在肿瘤细胞的普通非固缩核中也经常观察到强烈的TUNEL信号，偶尔也在对应于凋亡小体的核碎片中观察到。9例高分化癌的凋亡指数（凋亡细胞数/肿瘤细胞总数）为7.7 ~ 14.5%（平均10.9%），5例低分化癌的凋亡指数为2.7 ~ 7.5%（平均4.0%），前者的平均值明显高于后者（P
We have examined the occurrence of apoptotic cell death in formalin-fixed, paraffin-embedded human gastric carcinoma specimens by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) method. The specificity of the TUNEL signals was confirmed by the omission of either TdT or biotinylated dUTP as negative controls, and by pretreatment with DNase I as a positive control. Careful observation of routine hematoxylin and eosin-stained sections showed a few tumor cells with apoptosis, especially in well-differentiated carcinomas. Intense TUNEL signals were frequently observed even in ordinary, non-pyknotic nuclei of tumor cells, and occasionally also in nuclear fragments corresponding to apoptotic bodies. Apoptotic indices (number of apoptotic cells/total number of tumor cells) ranged between 7.7 and 14.5% (mean, 10.9%) in nine well-differentiated carcinomas and between 2.7 and 7.5% (mean, 4.0%) in five which were poorly differentiated, the mean number being significantly higher in the former (P