Applying Carbon Dots-Metal Ions Ensembles as a Multichannel Fluorescent Sensor Array: Detection and Discrimination of Phosphate Anions
Applying Carbon Dots-Metal Ions Ensembles as a Multichannel Fluorescent Sensor Array: Detection and Discrimination of Phosphate Anions
复制标题
应用碳点-金属离子整体作为多通道荧光传感器阵列：磷酸根阴离子的检测和辨别
DOI：
10.1021/acs.analchem.7b00602
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发表时间：
2017-05-16
影响因子：
7.4
通讯作者：
Lin, Hengwei
中科院分区：
文献类型：
--
作者：
Sun, Shan;Jiang, Kai;Lin, Hengwei
荧光碳点（CD）由于其上级光学性质和丰富的表面官能团，近年来在传感领域受到广泛关注。为了进一步利用这些独特的功能，CD被认为是可以方便地制造多通道传感器阵列。作为概念验证研究，CD-金属离子集合体在本研究中被筛选并设计为三通道荧光传感器阵列，用于识别各种磷酸根阴离子（例如，ATP，ADP，AMP，PPi和Pi）。进一步的研究表明，所选择的三种金属离子（即，Ce ~（3+）、Fe ~（3+）和Cu ~（2+）可诱导CD聚集，导致其荧光猝灭。然而，解聚集或进一步聚集的CD金属离子系综发生与另外的磷酸根阴离子。因此，CD的荧光正在恢复或进一步淬灭。由于不同数量的磷酸根基团和磷酸根阴离子的空间位阻效应，它们对传感器阵列的亲和力可以通过CD-金属离子系综的荧光变化来区分。通过统计分析方法，所开发的阵列显示出优异的性能，在检测和识别磷酸根阴离子。此外，通过对血清和盲样中磷酸盐的成功识别，验证了传感器阵列的实用性。与以前的报道相比，所开发的多通道传感器阵列表现出许多优点，如简单的制造工艺，灵活的调节检测范围，并可能扩展到具有类似化学结构或性质的其他分析物。
Fluorescent carbon dots (CDs) are attracting much attention in sensing recently thanks to their superior optical properties and abundant surface functional groups. To take further advantages of these unique features, CDs are considered to be possible for facilely fabricating multichannel sensor arrays. As a proof-of-concept research, CDs-metal ions ensembles are screened and designed as a triple-channel fluorescent sensor array in this study for the identification of various phosphate anions (e.g., ATP, ADP, AMP, PPi, and Pi) for the first time. Further studies reveal that the selected three metal ions (i.e., Ce3+, Fe3+, and Cu2+) could induce aggregation of the CDs, resulting in quenching of their fluorescence. However, disaggregation or further aggregation of the CDs metal ions ensembles occurs with the addition of phosphate anions. Consequently, fluorescence of the CDs is recovering or further quenching. On account of various numbers of phosphate group and steric hindrance effects of phosphate anions, their affinities to the sensor array can be distinguished through fluorescence changes of the CDs-metal ions ensembles. By means of statistical analysis methods, the as-developed array is shown excellent capabilities in the detection and discrimination of phosphate anions. Furthermore, practicability of the sensor array is validated by the successful identification of phosphates in serum and blind samples. Compared to previous reports, the as-developed multichannel sensor array manifests numerous advantages, such as simple fabrication process, flexible adjusting detection ranges, and possible extension to other analytes having similar chemical structures or properties.