Effects of acoustic wave resonance oscillation on immobilized enzyme
Effects of acoustic wave resonance oscillation on immobilized enzyme
复制标题
DOI：
10.1016/j.apsusc.2013.12.016
复制
发表时间：
2014-03
影响因子：
6.7
通讯作者：
H. Nishiyama;Tomoya Watanabe;Y. Inoue
中科院分区：
文献类型：
--
作者：
H. Nishiyama;Tomoya Watanabe;Y. Inoue
为了开发一种新的人工活化表面固定化酶催化剂的方法，研究了体声波的共振振荡效应。采用共价偶联的方法将葡萄糖氧化酶(GOD)固定在能够产生厚度-伸缩共振振荡(TERO)的铁电PZT器件上。在微型反应器中研究了GOD酶对葡萄糖的氧化作用。Tero的产生立即使固定化GOD的催化活性提高了2-3倍。随着Tero的关闭，活性没有明显下降，并且在反应进行过程中保持了80-90%的增强活性。当固定化的GOD暴露在无葡萄糖底物的溶液中时，几乎完全恢复到Tero产生之前的低水平。这些结果表明，葡萄糖底物的存在对于Tero诱导的GOD激活和保持增加的活性水平是必不可少的。反应温度、葡萄糖浓度、pH和射频功率对Tero活化的影响表明，Tero增强了固定化酶与葡萄糖底物的相互作用，从而促进了活化复合体的形成。
In aiming at developing a new method to artificially activate enzyme catalysts immobilized on surface, the effects of resonance oscillation of bulk acoustic waves were studied. Glucose oxidase (GOD) was immobilized by a covalent coupling method on a ferroelectric lead zirconate titanate (PZT) device that was able to generate thickness-extensional resonance oscillation (TERO). Glucose oxidation by the GOD enzyme was studied in a microreactor. The generation of TERO immediately increased the catalytic activity of immobilized GOD by a factor of 2–3. With turn-off of TERO, no significant activity decrease occurred, and 80–90% of the enhanced activity was maintained while the reaction proceeded. The almost complete reversion of the activity to the original low level before TERO generation was observed when the immobilized GOD was exposed to a glucose substrate-free solution. These results indicated that the presence of glucose substrate was essential for TERO-induced GOD activation and preservation of the increased activity level. The influences of reaction temperature, glucose concentration, pH, and rf electric power on the TERO activation showed that TERO strengthened the interactions of the immobilized enzyme with glucose substrate and hence promoted the formation of an activation complex.