Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells.
Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells.
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DOI：
10.1186/1471-213x-8-45
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发表时间：
2008-04-24
影响因子：
--
通讯作者：
Niwa H
中科院分区：
文献类型：
--
作者：
Masui S;Ohtsuka S;Yagi R;Takahashi K;Ko MS;Niwa H
Rex 1/Zfp 42已被广泛用作多能干细胞未分化状态的标志物。然而，它在多能干细胞，包括胚胎干细胞（ES）的功能仍然不清楚，虽然它参与内脏内胚层分化的F9胚胎癌（EC）细胞的报道。我们使用传统的基因打靶策略显示了Rex 1在小鼠ES细胞和胚胎中的功能。我们的研究结果清楚地表明，Rex 1的功能是在ES细胞和胚胎发育的多能性的维持。然而，Rex 1-/- ES细胞表现出缺陷，诱导内脏内胚层的标记基因的子集，当分化为胚状体时，如在EC细胞中所发现的。Rex 1仅作为多能性的标志物，而不像碱性磷酸酶活性那样具有功能意义。
Rex1/Zfp42 has been extensively used as a marker for the undifferentiated state of pluripotent stem cells. However, its function in pluripotent stem cells including embryonic stem (ES) cells remained unclear although its involvement in visceral endoderm differentiation in F9 embryonal carcinoma (EC) cells was reported. We showed the function of Rex1 in mouse ES cells as well as in embryos using the conventional gene targeting strategy. Our results clearly indicated that Rex1 function is dispensable for both the maintenance of pluripotency in ES cells and the development of embryos. However, Rex1-/- ES cells showed the defect to induce a subset of the marker genes of visceral endoderm, when differentiated as embryoid body, as found in EC cells. Rex1 should be regarded just as a marker of pluripotency without functional significance like the activity of alkaline phosphatase.