Effects of acoustic levitation on the development of zebrafish, Danio rerio, embryos.
Effects of acoustic levitation on the development of zebrafish, Danio rerio, embryos.
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DOI：
10.1038/srep13596
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发表时间：
2015-09-04
影响因子：
4.6
通讯作者：
Hæggström E
中科院分区：
文献类型：
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作者：
Sundvik M;Nieminen HJ;Salmi A;Panula P;Hæggström E
声波悬浮技术提供了在无壁环境中表征和操纵固体颗粒和流体等材料的潜力。虽然人们曾尝试让小动物悬浮起来，但这种悬浮的生物效应几乎没有记录在案。在这里，我们的目标是探索是否可以悬浮斑马鱼胚胎（压力节点和反节点的峰值压力分别为135 dB和144 dB）而对早期发育没有影响。我们将胚胎（n = 94）在受精后2-14小时（hpf）悬浮1000 （n = 47）或2000秒（n = 47）。我们比较了超声样本与对照组（n = 94）的主干神经突和耳石的大小和数量，发现差异无统计学意义（p < 0.05）。对照组死亡率（22.3%）低于1000 s悬浮组（34.0%）和2000 s悬浮组（42.6%），但差异无统计学意义（p < 0.05）。结果表明，小于2000秒的声悬浮不会影响斑马鱼胚胎的发育，但可能影响死亡率。声学悬浮可以作为一种非接触式无壁平台，用于表征和操纵椎骨胚胎，而不会对其发育造成重大不利影响。
Acoustic levitation provides potential to characterize and manipulate material such as solid particles and fluid in a wall-less environment. While attempts to levitate small animals have been made, the biological effects of such levitation have been scarcely documented. Here, our goal was to explore if zebrafish embryos can be levitated (peak pressures at the pressure node and anti-node: 135 dB and 144 dB, respectively) with no effects on early development. We levitated the embryos (n = 94) at 2–14 hours post fertilization (hpf) for 1000 (n = 47) or 2000 seconds (n = 47). We compared the size and number of trunk neuromasts and otoliths in sonicated samples to controls (n = 94), and found no statistically significant differences (p > 0.05). While mortality rate was lower in the control group (22.3%) compared to that in the 1000 s (34.0%) and 2000 s (42.6%) levitation groups, the differences were statistically insignificant (p > 0.05). The results suggest that acoustic levitation for less than 2000 sec does not interfere with the development of zebrafish embryos, but may affect mortality rate. Acoustic levitation could potentially be used as a non-contacting wall-less platform for characterizing and manipulating vertebrae embryos without causing major adverse effects to their development.