Analysis of Helper T Cell Responses to Cry j 1-Derived Peptides in Patients with Nasal Allergy: Candidate for Peptide-Based Immunotherapy of Japanese Cedar Pollinosis
Analysis of Helper T Cell Responses to Cry j 1-Derived Peptides in Patients with Nasal Allergy: Candidate for Peptide-Based Immunotherapy of Japanese Cedar Pollinosis
复制标题
DOI：
10.2332/allergolint.08-oa-0008
复制
发表时间：
2009-03-01
影响因子：
6.8
通讯作者：
Endo, Shuichiro
中科院分区：
文献类型：
--
作者：
Masuyama, Keisuke;Chikamatsu, Kazuaki;Endo, Shuichiro
背景：变应原特异性免疫治疗效果显著，但治疗过程中可能会发生不良反应。多肽免疫治疗已被认为是减少变态反应不良反应的新策略之一。方法：从12例日本雪松花粉症患者的外周血单个核细胞(PBMC)中建立12个Cry j 1特异性T细胞系。结果：与Cry j 1序列对应的4个多肽能够诱导多个T细胞系的增殖反应：p61-80(3/12；25.0%)；p115-132(2/12；16.6%)；p206-225(4/12；33.3%)；和p337-353(5/12，41.7%)。此外，来自12个捐赠者中的11个(91.7%)的T细胞系至少对这四种多肽中的一种有反应。另一方面，Cry j1特异性T细胞株产生的细胞因子模式也不同。结论：4种Cry j1多肽(p61-80、p115-132、p206-225和p337-353)可能是Cry j1分子的免疫优势T细胞表位，可用于雪松花粉症的多肽免疫治疗设计。
Background: Allergen specific immunotherapy is highly effective, but adverse events may occur during treatment. Peptide-based immunotherapy has been proposed as one of new strategies for reduction of allergic adverse reactions. We examined the possibility of candidate peptides for the development of peptide-based immunotherapy for Japanese cedar pollinosis.Methods: Twelve Cry j 1-specific T-cell lines were established from peripheral blood mononuclear cells (PBMC) of 12 patients with Japanese cedar pollinosis. Using these T-cell lines, 37 Cry j 1-derived overlapping peptides were assessed for their proliferative responses and cytokine production.Results: Four peptides corresponding to the Cry j 1 sequence were able to induce proliferative responses to more than one T-cell line: p61-80 (3/12; 25.0%); p115-132 (2/12; 16.6%); p206-225 (4/12; 33.3%); and p337-353 (5/12, 41.7%). Furthermore, T-cell lines generated from 11 of 12 donors (91.7%) responded to at least one of these four peptides. On the other hand, the pattern of cytokine production from Cry j 1-specific T-cell lines varied. Moreover, cytokine production patterns by stimulation with Cry j 1 peptide did not reflect those by stimulation with Cry j 1 protein.Conclusions: our results suggest four Cry j 1-derived peptides (p61-80, p115-132, p206-225 and p337-353) may be considered to be the immunodominant T-cell epitopes of the Cry j 1 molecule, and can be useful for the design of peptide-based immunotherapy for the management of Japanese cedar pollinosis.