Rapid simultaneous determination of cascade metabolites of acrylamide in urine for toxicokinetics profiles and short-term dietary internal exposure
Rapid simultaneous determination of cascade metabolites of acrylamide in urine for toxicokinetics profiles and short-term dietary internal exposure
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快速同时测定尿液中丙烯酰胺级联代谢物的毒代动力学特征和短期膳食内暴露
DOI：
10.1021/acs.jafc.0c01685
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发表时间：
2020
影响因子：
6.1
通讯作者：
Zhang Yu
中科院分区：
文献类型：
--
作者：
Zhang Yiju;Wang Qiao;Jia Wei;Cheng Jun;Zhu Li;Ren Yiping;Zhang Yu
本研究开发了一种超高效液相色谱串联质谱方法，可同时分析大鼠和人尿液中丙烯酰胺的级联代谢物，包括丙烯酰胺、缩水甘油酰胺、N-乙酰基-S-(2-氨基甲酰乙基)-L-半胱氨酸(AAMA)，N-乙酰基-S-(2-氨基甲酰基乙基)-l-半胱氨酸-亚砜，N-乙酰基-S-(2-氨基甲酰基-2-羟乙基)-l-半胱氨酸，和N-乙酰基-S-(1-氨基甲酰基-2-羟乙基)-l-半胱氨酸。串联固相萃取程序被新颖地用于从人尿液中一次性纯化所有代谢物。快速分析显示出高灵敏度，LOD 和 LOQ 范围分别为 0.1–0.8 和 0.4–5.8 ng/mL，并且每个样品在 8 分钟内实现了可接受的实验室内重现性 (RSD < 12.0%) 和加标回收率 (92.2%–117.3%)。在对雄性和雌性大鼠的尿液进行毒代动力学分析时，分别回收了约 70.7% 和 63.0% 的摄入丙烯酰胺。对于不吸烟的参与者，男性尿中丙烯酰胺和缩水甘油酰胺水平高于女性，而尿中 AAMA 浓度则表现出相反的行为。目前的分析为丙烯酰胺的级联代谢物的膳食短期内暴露评估提供了方法学支持。
The current study developed an ultrahigh-performance liquid chromatography tandem mass spectrometry method to simultaneously analyze cascade metabolites of acrylamide in urine of rats and humans, including acrylamide, glycidamide,N-acetyl-S-(2-carbamoylethyl)-l-cysteine (AAMA),N-acetyl-S-(2-carbamoylethyl)-l-cysteine-sulfoxide,N-acetyl-S-(2-carbamoyl-2-hydroxyethyl)-l-cysteine, andN-acetyl-S-(1-carbamoyl-2-hydroxyethyl)-l-cysteine. A tandem solid-phase extraction procedure was novelly used to purify all metabolites at once from human urine. The rapid analysis showed high sensitivity with LOD and LOQ ranges of 0.1–0.8 and 0.4–5.8 ng/mL, respectively, and achieved acceptable within-laboratory reproducibility (RSD < 12.0%) and spiking recovery (92.2%–117.3%) within 8 min per sample. Approximately 70.7 and 63.0% of ingested acrylamide were recovered during the toxicokinetics analysis from urine of male and female rats, respectively. For nonsmoking participants, the urinary levels of acrylamide and glycidamide were higher in men than women, whereas the urinary concentration of AAMA showed the opposite behavior. The current analysis provides methodological support of cascade metabolites of acrylamide for the dietary short-term internal exposure assessment of acrylamide.