A Vibrio cholerae protease needed for killing of Caenorhabditis elegans has a role in protection from natural predator grazing
A Vibrio cholerae protease needed for killing of Caenorhabditis elegans has a role in protection from natural predator grazing
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DOI：
10.1073/pnas.0601754103
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发表时间：
2006-06-13
影响因子：
11.1
通讯作者：
Wai, Sun Nyunt
中科院分区：
文献类型：
--
作者：
Vaitkevicius, Karolis;Lindmark, Barbro;Wai, Sun Nyunt
霍乱弧菌是腹泻病霍乱的致病细菌，其生长和存活被认为会受到纤毛虫和鞭毛虫等食菌捕食者的限制。在发现霍乱弧菌可以引起这种线虫的致命感染后，我们将秀丽隐杆线虫作为测试生物体。通过反向遗传学，我们确定了一种细胞外蛋白酶，即以前未表征的 PrtV 蛋白，它是杀伤所必需的。杀灭作用与秀丽隐杆线虫肠道内细菌的定植有关。我们还表明，PrtV 对于鞭毛虫 Cafeteria roenbergensis 和纤毛虫梨形四膜虫放牧后的霍乱弧菌生存至关重要。根据对人肠上皮细胞紧密单层的测试判断，PrtV 蛋白似乎在霍乱弧菌与哺乳动物宿主细胞的相互作用中具有间接作用。我们的结果证明了 PrtV 在霍乱弧菌与食草捕食者相互作用中的关键作用，并且我们将秀丽隐杆线虫确立为一种方便的生物体，用于鉴定参与宿主相互作用和环境持久性的霍乱弧菌因子。
Vibrio cholerae is the causal bacterium of the diarrheal disease cholera, and its growth and survival are thought to be curtailed by bacteriovorous predators, e.g., ciliates and flagellates. We explored Caenorhabditis elegans as a test organism after finding that V. cholerae can cause lethal infection of this nematode. By reverse genetics we identified an extracellular protease, the previously uncharacterized PrtV protein, as being necessary for killing. The killing effect is associated with the colonization of bacteria within the Caenorhabditis elegans intestine. We also show that PrtV is essential for V. cholerae in the bacterial survival from grazing by the flagellate Cafeteria roenbergensis and the ciliate Tetrahymena pyriformis. The PrtV protein appears to have an indirect role in the interaction of V. cholerae with mammalian host cells as judged from tests with tight monolayers of human intestinal epithelial cells. Our results demonstrate a key role for PrtV in V. cholerae interaction with grazing predators, and we establish Caenorhabditis elegans as a convenient organism for identification of V. cholerae factors involved in host interactions and environmental persistence.