COMPARISON OF ISOLATION AND CULTURE OF MESOPHYLL PROTOPLASTS FROM SEVERAL NICOTIANA SPECIES AND THEIR HYBRIDS
COMPARISON OF ISOLATION AND CULTURE OF MESOPHYLL PROTOPLASTS FROM SEVERAL NICOTIANA SPECIES AND THEIR HYBRIDS
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DOI：
10.1016/0304-4211(76)90162-0
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发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
EVANS, PK
中科院分区：
文献类型：
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作者：
BANKS, MS;EVANS, PK
从北美烟草、欧洲烟草、烟草及其杂交F1代中分离得到叶肉原生质体。Ootophora和N.sylvestris.Ootophora。在培养过程中，只有野油藻的植板率较低，但发现它的原生质体对光很敏感。从所有种和杂交种分离的原生质体来源的愈伤组织中获得了植株。
Mesophyll protoplasts were isolated from N. otophora, N. sylvestris, N. tabacum and the F1 hybrids N. tabacum .times. N. otophora and N. sylvestris .times. N. otophora. In culture only N. sylvestris had a low plating efficiency, although N. otophora protoplasts were found to be sensitive to light. Plants were recovered from callus tissues originating from isolated protoplasts of all the species and hybrids.