Screening of a fungus capable of powerful and selective delignification on wheat straw
Screening of a fungus capable of powerful and selective delignification on wheat straw
复制标题
一株对麦秆具有强效选择性脱木素作用的真菌的筛选
DOI：
10.1111/j.1472-765x.2008.02447.x
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发表时间：
2008-11-01
影响因子：
2.4
通讯作者：
Qu, Y. -B.
中科院分区：
文献类型：
--
作者：
Li, L.;Li, X. -Z.;Qu, Y. -B.
目的：为筛选和鉴定一株对小麦秸秆具有选择性脱木素能力的真菌，对一株在固态发酵（SSF）条件下对小麦秸秆具有高效脱木素能力的真菌进行了筛选。培养5天后，真菌降解去除了13.07%的木质素，损失了7.62%的综纤维素。液体发酵2d后，碱木质素的去除率为46.53%。根据其形态特征和18S rDNA基因序列分析，鉴定该菌为同色镰刀菌（Fusarium concolor）。用凝胶渗透色谱法测定了木质素的分子量分布。酶学分析表明，该菌在培养过程中产生漆酶、纤维二糖脱氢酶、木聚糖酶和纤维素酶。在液体发酵过程中产生木质素过氧化物酶、锰过氧化物酶和漆酶。研究结果表明，该菌株能有效地降解麦草中的木质素，对纤维素的损伤较小。研究意义：该菌株可用于生物制浆前的木质纤维素原料预处理、生物转化为燃料和化工基质。
Aims: To screen and characterize a novel fungus with powerful and selective delignification capability on wheat straw.Methods and Results: A fungus capable of efficient delignification under solid-state fermentation (SSF) conditions on wheat straw was screened. After 5 days of incubation, 13.07% of the lignin was removed by fungal degradation, and 7.62% of the holocellulose was lost. Furthermore, 46.53% of the alkali lignin was removed after 2 days of liquid fermentation. The fungus was identified as Fusarium concolor based on its morphology and an analysis of its 18S rDNA gene sequence. The molecular weight distribution of lignin was evaluated by gel permeation chromatography. Enzyme assay indicated that the fungus produced laccase, cellobiose dehydrogenase, xylanase and cellulase during the incubation period. Intracellular lignin peroxidase, manganese peroxidase and laccase were produced during liquid fermentation.Conclusions: We have successfully screened a fungus, F. concolor, which can efficiently degrade the lignin of wheat straw, with slight damage to the cellulose, after 5 days of SSF.Significance and Impact of the Study: The newly isolated strain could be used in pretreatment of lignocellulose materials prior to biopulping, bioconversion into fuel and substrates for the chemical industry.