Cloning and characterization of a putative inhibitor of melanization from Anopheles gambiae.
Cloning and characterization of a putative inhibitor of melanization from Anopheles gambiae.
复制标题
冈比亚按蚊黑化推定抑制剂的克隆和表征。
DOI：
10.1111/j.1365-2583.2006.00643.x
复制
发表时间：
2006
影响因子：
2.6
通讯作者：
Paskewitz,SM
中科院分区：
文献类型：
--
作者：
Shi,L;Li,B;Paskewitz,SM
Phenoloxidases, including tyrosinases and laccases, are enzymes involved in the synthesis of melanin, a process that can be elicited during insect immune responses, cuticle maturation, wound healing and egg chorion development. We cloned a putative inhibitor of melanization (POI) fromAnopheles gambiaeon the basis of homology with a functionally characterized peptide fromMusca domestica(Daquinaget al.,Proc Natl Acad Sci USA1995; 92: 2964–2968). The 335 amino acid protein predicted from theA. gambiaecDNA consists of five tandemly arranged inhibitor motifs. TheA. gambiaePOI gene was expressed in all mosquito stages from egg to adult. POI transcript levels were high in the fat body and were measurable but comparatively reduced in the midgut. The POI transcript level increased after wounding or Sephadex bead injection. Gene knockdown did not result in faster or more extensive bead melanization but did result in more extensive melanization of wound sites following a thoracic bead injection.
