Polymorphism of HLA class I genes in Meizhou Han population of Guangdong, China
Polymorphism of HLA class I genes in Meizhou Han population of Guangdong, China
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DOI：
10.1111/j.1744-313x.2007.00669.x
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发表时间：
2007-04-01
影响因子：
2.2
通讯作者：
Xu, A.
中科院分区：
文献类型：
--
作者：
Chen, S.;Li, W.;Xu, A.
人类白细胞抗原（HLA）因其极端的多态性而成为人类学研究的宝贵标记。在中国人群中进行的研究大多是针对HLA II类基因，而针对I类基因的研究很少。在本研究中，我们采用聚合酶链式反应测序分型（PCR-SBT）方法对中国南方广东梅州的 104 名无血缘关系的汉族个体进行了 HLA I 类多态性研究。鉴定了 23 个 HLA-A、43 个 HLA-B 和 27 个 HLA-C 等位基因，并对等位基因频率以及二位点 (C/B) 和三位点 (A/C/B) 单倍型进行了统计分析。最常见的 HLA-A 等位基因是 A*110101，频率为 30.3%，其次是 A*24020101 (22.2%) 和 A*2420 (11.6%)。在检测到的 43 个 HLA-B 等位基因中，频繁观察到 B*5801（17.0%）、B*400101（15.5%）和 B*4601（10.0%）。在检测到的 27 个 C 等位基因中，最主要的一个是 Cw*07020101 (25.8%)，其次是 Cw*0717 (14.7%)。最常见的 HLA-C/B 双基因座单倍型是 Cw*07020101/B*400101 (10.1%)。梅州汉族最常见的HLA-A/C/B三基因座单倍型是A*110101/Cw*07020101/B*400101（3.4%）。基于 HLA I 类等位基因频率的系统发育树从遗传学角度表明梅州汉族与南亚人群有亲缘关系。这一结果也可能反映出汉族和中国南方少数民族的混合。
Human leukocyte antigen (HLA) is an invaluable marker for anthropological studies because of its extreme polymorphism. Most of the studies carried out in Chinese populations are about HLA class II genes, but few about class I genes. In the present study, we investigated HLA class I polymorphism using polymerase chain reaction-sequencing-based typing (PCR-SBT) method in 104 unrelated Han individuals in Meizhou of Guangdong, southern China. Twenty-three HLA-A, 43 HLA-B and 27 HLA-C alleles were identified and allele frequencies and two-locus (C/B) and three-locus (A/C/B) haplotypes were statistically analysed. The most frequent HLA-A allele is A*110101 with a frequency of 30.3%, followed by A*24020101 (22.2%) and A*2420 (11.6%). Among the 43 detected HLA-B alleles, B*5801 (17.0%), B*400101 (15.5%) and B*4601 (10.0%) were frequently observed. Among the 27 detected C alleles, the most predominant one is Cw*07020101 (25.8%), followed by Cw*0717 (14.7%). The most frequent HLA-C/B two-locus haplotype is Cw*07020101/B*400101 (10.1%). The most common HLA-A/C/B three-locus haplotype in Meizhou Han is A*110101/Cw*07020101/B*400101 (3.4%). Phylogenetic tree based on HLA class I allele frequencies genetically suggested that Meizhou Han has an affinity to southern Asian populations. The result may also reflect an admixture of Han and ethnic minorities of southern China.