Induction of advanced glycation end products and alterations of the tensile properties of articular cartilage
Induction of advanced glycation end products and alterations of the tensile properties of articular cartilage
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DOI：
10.1002/art.10627
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发表时间：
2002-12-01
影响因子：
--
通讯作者：
Sah, RL
中科院分区：
文献类型：
--
作者：
Chen, AC;Temple, MM;Sah, RL
目标。确定将牛关节软骨中的晚期糖基化终产物（AGEs）增加到老年人类软骨中的水平是否会调节组织的拉伸生物力学特性。成年牛关节软骨样品在含有核糖的缓冲溶液中孵育以诱导AGEs的形成或在对照溶液中孵育。对部分软骨样品进行AGEs的生化指标分析，并测试其拉伸生物力学性能，包括刚度、强度和断裂伸长率。其软骨处理诱导组织荧光、吸光度和戊苷含量的增加（每次比较P < 0.001），其数量与人类衰老过程中发生的数量相似。核糖处理还能使软骨的动模量（增加60%）和强度（增加35%）增加，应变（减少25%）减少（P < 0.001）。在核糖处理后，AGEs的增加和软骨拉伸性能的改变表明，年龄相关的AGEs变化对该组织有功能影响。年龄相关的软骨强度和刚度的增加可能是有益的，因为它抵消了与退变相关的这些特性的降低。相反，与年龄相关的失效长度的减少，脆性的增加，以及刚度的增加，可能使软骨更易发生应力集中、骨折和与年龄相关的生物力学功能障碍。
Objective. To determine whether increasing advanced glycation end products (AGEs) in bovine articular cartilage to levels present in aged human cartilage modulates the tensile biomechanical properties of the tissue.Methods. Adult bovine articular cartilage samples were incubated in a buffer solution with ribose to induce the formation of AGEs or in a control solution. Portions of cartilage samples were assayed for biochemical indices of AGEs and tested to assess their tensile biomechanical properties, including stiffness, strength, and elongation at failure.Results. Whose treatment of cartilage induced increases in tissue fluorescence, absorbance, and pentosidine content (P < 0.001 for each comparison) by amounts similar to those that occur during aging in humans. Ribose treatment of cartilage also induced an increase in dynamic modulus (60% increase) and strength (35% increase), and a decrease (25% decrease) in strain (P < 0.001 for each comparison).Conclusion. The concomitant increase in AGEs and alteration of tensile properties of cartilage after ribose treatment suggest that aging-associated changes in AGEs have functional consequences for this tissue. The AGE-associated increases in strength and stiffness of cartilage may be beneficial by counteracting the decreases in these properties that are associated with degeneration. Conversely, the AGE-associated decrease in failure length, or increase in brittleness, together with increased stiffness may predispose cartilage to increased stress concentration, fracture, and aging-associated biomechanical dysfunction.