Removal of repressive histone marks creates epigenetic memory of recurring heat in Arabidopsis
Removal of repressive histone marks creates epigenetic memory of recurring heat in Arabidopsis
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DOI：
10.1101/2020.05.10.086611
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发表时间：
2020-05
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通讯作者：
Nobutoshi Yamaguchi;Satoshi Matsubara;Kaori Yoshimizu;Motohide Seki;K. Hamada;Mari Kamitani;Y. Kurita;Soichi Inagaki;Takamasa Suzuki;Eng-Seng Gan;T. To;T. Kakutani;A. Nagano;A. Satake;Toshiro Ito
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作者：
Nobutoshi Yamaguchi;Satoshi Matsubara;Kaori Yoshimizu;Motohide Seki;K. Hamada;Mari Kamitani;Y. Kurita;Soichi Inagaki;Takamasa Suzuki;Eng-Seng Gan;T. To;T. Kakutani;A. Nagano;A. Satake;Toshiro Ito
适应高温会增加植物对随后致命高温的耐受力1-3。尽管植物基因表达的表观遗传调控已得到充分研究，但植物如何随着时间的推移保持环境变化的记忆仍不清楚。在这里，我们表明 JUMONJI (JMJ) 蛋白 4-8，即参与组蛋白 H3 赖氨酸 27 三甲基化 (H3K27me3) 的去甲基化酶，是拟南芥热驯化所必需的。驯化过程通过 JMJ 诱导关键热休克蛋白 (HSP) 位点持续 H3K27me3 去甲基化，为 HSP 基因随后的激活做好准备。 3 天间隔后感应到热量后，JMJ 会直接重新激活 HSP 基因。最后，jmj 突变体在波动的田间温度条件下无法维持热记忆。我们对涉及组蛋白去甲基酶的表观遗传记忆机制的发现可能对田间植物的环境适应具有影响。
Acclimation to high temperature increases plants’ tolerance of subsequent lethal high temperatures1-3. Although epigenetic regulation of plant gene expression is well studied, how plants maintain a memory of environmental changes over time remains unclear. Here, we show that JUMONJI (JMJ) proteins4-8, demethylases involved in histone H3 lysine 27 trimethylation (H3K27me3), are necessary for Arabidopsis thaliana heat acclimation. Acclimation induces sustained H3K27me3 demethylation at key HEAT SHOCK PROTEIN (HSP) loci by JMJs, poising the HSP genes for subsequent activation. Upon sensing heat after a 3-day interval, JMJs directly reactivate HSP genes. Finally, jmj mutants fail to maintain heat memory under fluctuating field temperature conditions. Our findings of an epigenetic memory mechanism involving histone demethylases may have implications for environmental adaptation of field plants.