Protein, enzyme and carbohydrate quantification using smartphone through colorimetric digitization technique
Protein, enzyme and carbohydrate quantification using smartphone through colorimetric digitization technique
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DOI：
10.1002/jbio.201500329
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发表时间：
2017-05-01
影响因子：
2.8
通讯作者：
Nath, Pabitra
中科院分区：
文献类型：
--
作者：
Dutta, Sibasish;Saikia, Gunjan Prasad;Nath, Pabitra
本文介绍了利用Smartphone作为生物大分子比色定量的检测平台。利用HSV颜色空间的V通道，成功地研究了BSA蛋白、过氧化氢酶和碳水化合物(使用D-葡萄糖)的定量。已经开发了一个定制设计的Android应用程序，用于估计生物大分子的总浓度。结果已与标准分光光度计的结果进行了比较，标准分光光度计通常用于实验室环境中通过测量其在特定波长的吸光度进行比色定量。将所设计的传感器与分光光度计测得的数据进行了比较，结果基本一致。所设计的传感器成本低、坚固耐用，我们预计它将促进生物分析研究的各个领域。
In this paper the utilization of smartphone as a detection platform for colorimetric quantification of biological macromolecules has been demonstrated. Using V-channel of HSV color space, the quantification of BSA protein, catalase enzyme and carbohydrate (using D-glucose) have been successfully investigated. A custom designed android application has been developed for estimating the total concentration of biological macromolecules. The results have been compared with that of a standard spectrophotometer which is generally used for colorimetric quantification in laboratory settings by measuring its absorbance at a specific wavelength. The results obtained with the designed sensor is found to be similar when compared with the spectrophotometer data. The designed sensor is low cost, robust and we envision that it could promote diverse fields of bio-analytical investigations.