Autologous apoptotic cell engulfment stimulates chemokine secretion by vascular smooth muscle cells.
Autologous apoptotic cell engulfment stimulates chemokine secretion by vascular smooth muscle cells.
复制标题
自体凋亡细胞吞噬刺激血管平滑肌细胞分泌趋化因子。
DOI：
10.1016/s0002-9440(10)62980-x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Ischiropoulos,Harry
中科院分区：
文献类型：
--
作者：
Fries,DianaM;Lightfoot,Richard;Koval,Michael;Ischiropoulos,Harry
Apoptosis of vascular smooth muscle cells (VSMCs) occurs in vivo under both physiological and pathological settings. The clearance of apoptotic cells may be accomplished in part by the surrounding normal VSMCs. However, the fate of internalized apoptotic cells, the rate of intracellular degradation, and the consequences of these processes to VSMC biology are unknown. Electron microscopy and confocal fluorescence imaging showed that rat VSMCs effectively bound and internalized autologous apoptotic VSMCs in vitro. Within 2 hours, the internalized apoptotic cells were delivered to lysosomes, and the majority of these internalized cells and their proteins were efficiently degraded by 24 hours. After degradation was completed, the phagocytic VSMCs remained viable with normal rates of proliferation. Clearance of apoptotic cells by VSMCs did not induce the release of vascular wall matrix proteases but was associated with a 1.6-fold increase in transforming growth factor-β1 release. Interestingly, clearance of apoptotic cells stimulated VSMCs to secrete monocyte-chemoattractant protein-1 and cytokine-induced neutrophil chemoattractant. The coordinated release of transforming growth factor-β1 and chemokines suggests that autologous apoptotic cell clearance stimulates VSMCs to release molecules that specifically recruit professional phagocytes while simultaneously dampening the inflammatory response and preventing vascular injury.
