A promising self-nanoemulsifying adjuvant with plant-derived saponin D boosts immune response and exerts an anti-tumor effect.
A promising self-nanoemulsifying adjuvant with plant-derived saponin D boosts immune response and exerts an anti-tumor effect.
复制标题
植物源性皂苷D的自纳米乳化佐剂具有增强免疫反应和抗肿瘤作用的前景。
DOI：
10.3389/fimmu.2023.1154836
复制
发表时间：
2023
影响因子：
7.3
通讯作者：
Zou, Quanming
中科院分区：
文献类型：
--
作者：
Luo, Xing;Song, Zhen;Zeng, Xiaogqiang;Ye, Yan;Zheng, Hailin;Cai, Dingyi;Yuan, Qingpeng;Li, Haibo;Tong, Yanan;Lu, Dongshui;Liu, Yuheng;Zeng, Hao;Yang, Yun;Sun, Hongwu;Zou, Quanming
肿瘤抗原的低免疫原性和佐剂的不可接受的毒性阻碍了肿瘤疫苗的应用和发展。因此，我们设计了一种由植物源性免疫刺激分子纳米佐剂（一种自纳米乳化系统，SND）和抗原OVA组成的新型抗肿瘤疫苗，以重新激活免疫反应并抑制肿瘤进展。本研究设计并采用低能乳化法制备了新型皂苷D （SND）纳米佐剂。研究了SND的几个重要特性，包括形态、大小、聚合物分散指数（PDI）、zeta电位和稳定性，并通过MTT法评估了SND的细胞毒性。此外，我们还在体内评估了疫苗免疫后的免疫反应，包括抗体滴度水平和细胞免疫，并评估了这种新型疫苗对肿瘤的预防和治疗效果。最后，通过IVIS成像和体内实验确定抗原释放谱。该SND纳米佐剂的平均粒径为26.35±0.225 nm，粒径分布为0.221±1.76，zeta电位为-12.9±0.83 mV，具有良好的稳定性。具有良好的稳定性（粒径、PDI、zeta电位、抗原稳定性），体内外毒性低，体内抗原释放延迟。免疫0、14、28 d后，小鼠体液免疫应答（IgG、IgG1、IgG2a和IgG2b）和细胞免疫水平（脾细胞因子包括IFN-γ、IL-4、IL-1β和il - 17a）均有显著提高。重要的是，这种新型纳米佐剂与OVA联合使用可能会诱导E.G7-OVA荷瘤小鼠的预防和治疗效果。这些结果表明，这种新型纳米佐剂包封的天然植物免疫刺激分子OPD可能是一种很好的候选肿瘤疫苗佐剂，具有激活免疫反应和有效抑制肿瘤生长的作用。
The low immunogenicity of tumor antigens and unacceptable toxicity of adjuvants has hindered the application and development of tumor vaccines. Hence, we designed a novel anti-tumor vaccine composed of a plant-derived immunostimulant molecular nanoadjuvant (a self-nanoemulsifying system, SND) and the antigen OVA, to reinvigorate the immune response and inhibit tumor progression. In this study, this novel nanoadjuvant with Saponin D (SND) was designed and prepared by low-energy emulsification methods. Several important characteristics of the SND, including morphology, size, polymer dispersity index (PDI), zeta potential, and stability, were estimated, and the cytotoxicity of the SND was evaluated by MTT assay. Additionally, the immune response in terms of antibody titer levels and cellular immunity were evaluated in vivo after immunization with the vaccine, and the preventative and therapeutic effects of this novel vaccine against tumors were estimated. Finally, the antigen release profile was determined by IVIS imaging and by in vivo assay. This SND nanoadjuvant had good characteristics including the average particle size of 26.35 ± 0.225 nm, narrow distribution of 0.221 ± 1.76, and stability zeta potential of -12.9 ± 0.83 mV. And also, it had good stability (size, PDI, zeta potential, antigen stability) and low toxicity in vitro and in vivo, and delayed antigen release in vivo. The humoral immune response (IgG, IgG1, IgG2a, and IgG2b) and cellular immune level (cytokines of splenocytes including IFN-γ, IL-4, IL-1β andIL-17A) were both improved greatly after injected immunization at 0, 14, 28 days with the novel nanoadjuvant and antigen OVA. Importantly, this novel nanoadjuvant combined with OVA might lead to the induction of the prevent and treatment efficacy in the E.G7-OVA tumor-bearing mice. These results suggested that this novel nanoadjuvant encapsulated natural plant immunostimulant molecular OPD could be a good candidate of tumor vaccine adjuvant for reinvigorating the immune response and powerfully inhibiting tumor growth effect.
登录
查看更多内容
开发安全有效的藤黄酸纳米乳递送系统，用于体外和体内治疗急性髓系白血病
影响因子：
4.6
作者：
Feng, Ziqi;Wang, Zelin;Sun, Hongwu
通讯作者：
Sun, Hongwu
影响因子：
7.2
作者：
Dadaglio, Gilles;Fayolle, Catherine;Leclerc, Claude
通讯作者：
Leclerc, Claude
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1016/j.ijbiomac.2020.02.275
发表时间：
2020-06-01
影响因子：
8.2
作者：
Batool, Ayesha;Arshad, Rabia;Shahnaz, Gul
通讯作者：
Shahnaz, Gul
影响因子：
--
作者：
Cai, Dingyi;Zhang, ZeLong;Sun, Hongwu
通讯作者：
Sun, Hongwu