Monitoring of enterovirus diversity in wastewater by ultra-deep sequencing: An effective complementary tool for clinical enterovirus surveillance
Monitoring of enterovirus diversity in wastewater by ultra-deep sequencing: An effective complementary tool for clinical enterovirus surveillance
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DOI：
10.1016/j.watres.2019.115246
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发表时间：
2020-02-01
期刊：
影响因子：
12.8
通讯作者：
Henquell, Cecile
中科院分区：
文献类型：
--
作者：
Bisseux, Maxime;Debroas, Didier;Henquell, Cecile
在一项为期一年（2014年10月至2015年10月）的试点研究中，我们评估了废水监测与持续采样，以分析城市社区的全球肠道病毒（EV）感染。在PCR扩增部分VP1衣壳蛋白基因后，通过超深度测序（UDS）分析废水。核苷酸序列分析表明，该群落中存在48个EV类型，其中A（n = 13）、B（n = 23）和C（n = 12）是该群落中最丰富的EV类型。在同一时期，在为同一城市人口服务的教学医院转诊的患者中确定了26种EV类型，其中22种在废水中检测到。废水监测检测到26种EV类型的无声循环，包括临床罕见呼吸道疾病中报告的病毒。废水监测作为一种补充程序，可以补充与非脊髓灰质炎EV相关的严重疾病的临床监测，并有助于根除脊髓灰质炎的最后阶段。(C)2019年，任作家。爱思唯尔有限公司出版
In a one-year (October 2014-October 2015) pilot study, we assessed wastewater monitoring with sustained sampling for analysis of global enterovirus (EV) infections in an urban community. Wastewater was analysed by ultra-deep sequencing (UDS) after PCR amplification of the partial VP1 capsid protein gene. The nucleotide sequence analysis showed an unprecedented diversity of 48 EV types within the community, which were assigned to the taxonomic species A (n = 13), B (n = 23), and C (n = 12). During the same period, 26 EV types, of which 22 were detected in wastewater, were identified in patients referred to the teaching hospital serving the same urban population. Wastewater surveillance detected a silent circulation of 26 EV types including viruses reported in clinically rare respiratory diseases. Wastewater monitoring as a supplementary procedure can complement clinical surveillance of severe diseases related to non-polio EVs and contribute to the final stages of poliomyelitis eradication. (C) 2019 The Authors. Published by Elsevier Ltd.