CD25 expression on donor CD4+ or CD8+ T cells is associated with an increased risk for graft-versus-host disease after HLA-identical stem cell transplantation in humans
CD25 expression on donor CD4+ or CD8+ T cells is associated with an increased risk for graft-versus-host disease after HLA-identical stem cell transplantation in humans
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DOI：
10.1182/blood-06-2085
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发表时间：
2004-02-01
期刊：
影响因子：
20.3
通讯作者：
Komanduri, KV
中科院分区：
文献类型：
--
作者：
Stanzani, M;Martins, SLR;Komanduri, KV
移植物抗宿主病（GVHD）在30%至50%的匹配相关移植后以不可预测的方式发生。在小鼠模型中，供体移植物中CD 4（+）CD 25（+）调节性T细胞频率增加已被证明可改善同种异体移植后的GVHD。为了确定在人类中是否存在类似的关系，我们定量了60例输注到匹配同胞中的供体移植物中CD 4（+）和CD 8（+）T细胞上CD 25的共表达，并检查了各自受体中GVHD的发生率。发生GVHD的受者接受的供体移植物中共表达CD 25的CD 4 + T细胞频率明显高于未发生GVHD的受者（中位数，9.26% vs 2.22%; P = 0.004）。共表达CD 25的供体移植物CD 8 + T细胞的频率也较高（0.65% vs 0.14%; P =.002）。此外，接受含有较少CD 4 + CD 25+和CD 8（+）CD 25（+）T细胞的移植物的移植受者不太可能获得急性GVHD，即使这些供体-受体对在相关临床变量方面与其他供体相似。这些数据表明，CD 4和CD 25的共表达可能不足以识别人类中的调节性T细胞，并且供体移植物中CD 25（+）T细胞的频率和数量增加与移植受体中的GVHD相关。
Graft-versus-host disease (GVHD) occurs in an unpredictable fashion after 30% to 50% of matched-related transplantations. The presence of increased frequencies of CD4(+)CD25(+) regulatory T cells in donor grafts has been shown to ameliorate GVHD after allogeneic transplantation in murine models. To determine whether a similar relationship exists in humans, we quantitated the coexpression of CD25 on CD4(+) and CD8(+) T cells within 60 donor grafts infused into matched siblings and examined GVHD incidence in the respective recipients. Recipients in whom GVHD developed received donor grafts containing significantly higher frequencies of CD4+ T cells coexpressing CD25 than those who did not (median, 9.26% vs 2.22%; P =.004). Frequencies of donor graft CD8+ T cells coexpressing CD25 were also higher (0.65% vs 0.14%; P =.002). Furthermore, transplant recipients who received grafts containing fewer CD4+CD25+ and CD8(+)CD25(+) T cells were less likely to acquire acute GVHD, even though these donor-recipient pairs were similar to others with respect to relevant clinical variables. These data suggest that the coexpression of CD4 and CD25 may be insufficient to identify regulatory T cells in humans and that increased frequencies and numbers of CD25(+) T cells in donor grafts is associated with GVHD in transplant recipients.